Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Nuclear pattern of CXCR4 expression is associated with a better overall survival in patients with gastric cancer

(2014) Nuclear pattern of CXCR4 expression is associated with a better overall survival in patients with gastric cancer. Journal of Oncology.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction. Previous studies have shown that stromal-derived factor-1 (CXCL12) and its receptor, CXCR4, play a crucial role in metastasis of various tumors. Similarly, it has been cleared that CXCR4 is expressed on the cell surface of gastric cancers. However, nuclear expression of CXCR4 and its clinical importance have not been yet studied. Materials and Methods. Herein, we studied the expression of CXCR4 in gastric samples from patients with gastric adenocarcinoma as well as human gastric carcinoma cell line, AGS, by employing RT-PCR, immunohistochemistry, and flow cytometry techniques. Results. RT-PCR data showed that CXCR4 is highly expressed on AGS cells. This was confirmed by IHC and FACS as CXCR4 was detected on cell membrane, in cytoplasm, and in nucleus of AGS cells. Moreover, we found that both cytoplasmic and nuclear CXCR4 are strongly expressed in primary gastric cancer and the cytoplasmic pattern of CXCR4 tends to be associated with a shorter overall survival than nuclear staining. In conclusion, we present evidence for the first time that both cytoplasmic and nuclear expression of CXCR4 are detectable in gastric cancer tissues. However, the role of both cytoplasmic and nuclear CXCR4 needs to be further elucidated. © 2014 Bahram Nikkhoo et al.

Item Type: Article
Keywords: chemokine receptor CXCR4, aged; article; cancer cell culture; cancer patient; cancer survival; cell membrane; cell surface; cytoplasm; female; flow cytometry; human; human cell; immunohistochemistry; male; overall survival; protein analysis; protein expression; reverse transcription polymerase chain reaction; stomach adenocarcinoma
Journal or Publication Title: Journal of Oncology
Publisher: Hindawi Publishing Corporation
Identification Number: 10.1155/2014/808012
ISSN: 16878450
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/1002

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