(2014) Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line. Research in Pharmaceutical Sciences.
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Abstract
Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 μM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A (p>0.05); whereas p21 and Bax expression were significantly (p<0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A.
Item Type: | Article |
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Keywords: | disulfiram; messenger RNA; protein Bax; protein p21; Ras association domain family protein 1A, antineoplastic activity; apoptosis; article; cancer cell culture; controlled study; human; human cell; methylation; pancreas cancer; real time polymerase chain reaction |
Page Range: | pp. 287-294 |
Journal or Publication Title: | Research in Pharmaceutical Sciences |
Volume: | 9 |
Number: | 4 |
Publisher: | Isfahan University of Medical Sciences(IUMS) |
ISSN: | 17355362 |
Depositing User: | مهندس جمال محمودپور |
URI: | http://eprints.muk.ac.ir/id/eprint/1032 |
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