Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

No detection of xenotropic murine leukemia virus-related viruses in prostate cancer in Sanandaj, West of Iran

(2013) No detection of xenotropic murine leukemia virus-related viruses in prostate cancer in Sanandaj, West of Iran. Asian Pacific Journal of Cancer Prevention.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Multiple etiologies have been hypothesized for prostate cancer, including genetic defects and infectious agents. A recently reported gamaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) has been reported to be detected in prostate cancer. However, this virus has not been detected in similar groups of patients in other studies. Herein, we sought to detect XMRV in prostate cancers and benign controls in Sanandaj, west of Iran. Materials and Methods: In a case-control study, genomic DNA was extracted from formalin fixed and paraffin embedded prostate tissues from a total of 163 Iranian patients. We developed a conventional and a nested PCR assay using primers targeting to an env specific sequence of XMRV. PCR assays were carried out on 63 prostate cancers and 100 benign prostate hyperplasias. Results: Beta-actin sequences were successfully detected in the DNA extracts from all prostate tissues, confirming DNA extraction integrity. We did not detect XMRV in samples either from prostate cancers or benign prostate hyperplasias using XMRV specific primers. Conclusions: We conclude that in our population XMRV does not play a role in genesis of prostate cancer.

Item Type: Article
Keywords: virus DNA, adenocarcinoma; adult; aged; article; cancer grading; follow up; genetics; human; Iran; isolation and purification; male; middle aged; pathogenicity; polymerase chain reaction; prognosis; prostate; prostate tumor; retrovirus infection; very elderly; virology; Xenotropic MuLV related virus, Adenocarcinoma; Adult; Aged; Aged, 80 and over; DNA, Viral; Follow-Up Studies; Humans; Iran; Male; Middle Aged; Neoplasm Grading; Polymerase Chain Reaction; Prognosis; Prostate; Prostatic Neoplasms; Retroviridae Infections; Xenotropic murine leukemia virus-related virus
Page Range: pp. 6929-6933
Journal or Publication Title: Asian Pacific Journal of Cancer Prevention
Volume: 14
Number: 11
Identification Number: 10.7314/APJCP.2013.14.11.6929
ISSN: 15137368
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/1240

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