Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

In vitro differentiation of human bone marrow-derived mesenchymal stem cells into cardiomyocyte-like cells

(2010) In vitro differentiation of human bone marrow-derived mesenchymal stem cells into cardiomyocyte-like cells. Yakhteh.

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Objective: Human mesenchymal stem cells (MSCs) have been recognized as potential candidates for cell therapy. In the present study, the ability of human bone marrow mesenchymal stem cells (hBMSCs) to differentiate into cells with characteristics of cardiomyocytes in vitro was investigated. Materials and Methods: hBMSCs cultured in enriched medium were treated with oxytocin and 5-azacytidin. The differentiation of hBMSCs into cells that expressed cardiacspecific genes such as α3-actinin, alpha - myosin heavy chain (α-MHC), beta - myosin heavy chain (β-MHC), myosin light chain isoform 2a (MLC2a), myosin light chain isoform 2v (MLC2v), artial natriuretic factor (ANF), GATA4 and oxytocin receptor (OTR) was investigated by reverse transcription-polymerase chain reaction (RT-PCR). Protein expressions of β-actinin and troponin l-C in the cells were analyzed through immunofluorescence staining. Results: MSCs are spindle-shaped with irregular processes. Cells treated with oxytocin and 5-azacytidin connected with adjoining cells to form myotube-like structures. Expressions of a number of cardiac-specific genes were detected by RT-PCR. Immunofluorescence staining analysis showed that the differentiated cells stained positively for β-actinin and troponin l-C protein. Conclusion: These results indicate that adult hBMSCs can differentiate into cardiomyocytes in vitro by treatment with oxytocin and 5-azacytidin, and can be considered as a source of cells for cellular cardiomyoplasty.

Item Type: Article
Keywords: alpha actinin 3; atrial natriuretic factor; azacitidine; beta actin; myosin heavy chain alpha; myosin heavy chain beta; myosin light chain; myosin light chain 2a; myosin light chain 2v; oxytocin; oxytocin receptor; transcription factor GATA 4; troponin C; troponin I; unclassified drug, article; bone marrow cell; cell culture; cell differentiation; gene expression; heart muscle cell; human; human cell; immunofluorescence test; in vitro study; mesenchymal stem cell; protein expression; reverse transcription polymerase chain reaction
Page Range: pp. 387-394
Journal or Publication Title: Yakhteh
Volume: 12
Number: 3
ISSN: 15614921
Depositing User: مهندس جمال محمودپور

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