Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Genotoxicity study of Hypiran and Chamomilla herbal drugs determined by in vivo supervital micronucleus assay with mouse peripheral reticulocytes

(2009) Genotoxicity study of Hypiran and Chamomilla herbal drugs determined by in vivo supervital micronucleus assay with mouse peripheral reticulocytes. Acta Biologica Hungarica.

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Abstract

The growth in popularity of Over The Counter (OTC) of medicinal products or other natural sources have taken a very large share of healthcare system therefore it is essential to determine their safety as for as public health is concerned.In this study Maximum Tolerated Dose (MTD) was obtained according to CSGMT protocol presented by the Environmental Mutagen Society of Japan. The positive group received mitomycin C in dose of 0.5 mg/kg. The peripheral blood samples before treatment (zero time) were considered as negative control. The MTD of Hypiron was 12 ml/kg and for Chamomilla was 10 ml/kg. Doses of MTD, 1/2 MTD and 1/4 MTD were considered for test groups. Then blood samples were prepared 48 hours after first administration of drugs and kept on precoated Acridine orange slides. The scoring of micronucleated reticulocytes were carried out per 2000 counted reticulocytes in each slide by fluorescent microscope. The results were statistically analyzed. Results of Hypiran were compared with negative control group and the P value was P > 0.05, but the p value of Chamomilla was P < 0.05. Also, the p value of Hypiran and Chamomilla compared with historical negative control group was less, therefore Chamomilla herbal drog can be classified as equivocul category of genotoxicity and Hypiran did not show genotoxicity. © 2009 Akadémiai Kiadó, Budapest.

Item Type: Article
Keywords: acridine; acridine orange; chamomile; environmental mutagen; herbaceous agent; hypiran; mitomycin C; unclassified drug, animal cell; animal experiment; article; blood sampling; concentration response; controlled study; fluorescence microscope; fluorescence microscopy; genotoxicity; in vivo study; male; maximum tolerated dose; micronucleus test; mouse; nonhuman; reticulocyte; reticulocyte count, Alkylating Agents; Animals; Biological Assay; Dose-Response Relationship, Drug; Maximum Tolerated Dose; Mice; Micronucleus Tests; Microscopy, Fluorescence; Mitomycin; Mutagenesis; Plant Extracts; Plant Preparations; Reticulocytes; Time Factors, Chamomilla
Page Range: pp. 177-183
Journal or Publication Title: Acta Biologica Hungarica
Volume: 60
Number: 2
Identification Number: 10.1556/ABiol.60.2009.2.5
ISSN: 02365383
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/1595

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