Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Role of stromal derived factor-1a (SDF-1a) for spermatogenesis of busulfan-injured rats

(2017) Role of stromal derived factor-1a (SDF-1a) for spermatogenesis of busulfan-injured rats. Reproductive Toxicology.

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Abstract

SDF-1a is a member of CXC chemokine family that plays a crucial role in stem cell migration, cell apoptosis and development. The role of intra-scrotal administration of SDF-1a in spermatogenesis of busulfan-treated rats was investigated in this study. Two injections of busulfan (15 mg/kg) with a 14 days interval between were given intraperitoneally to male Wistar rats. Rats were then treated for seven days with 500 ng/mL SDF-1a. Real-time PCR and immunohistochemistry were performed for evaluation of various cell markers for proliferation and spermatogenesis, and sperm parameters were assessed. In the SDF-1a group, there was a significant increase in testis weight, sperm count and viability. DAZL, DDX4, and TP2 showed increased expression levels in the SDF-1a group. PCNA and BrdU revealed highest expression rates in the SDF-1a group (p ≤ 0.0001). These findings showed the protective role of SDF-1a in busulfan-induced testis injury most likely through stimulation of SSCs proliferation. © 2017 Elsevier Inc.

Item Type: Article
Keywords: broxuridine; busulfan; cycline; DEAD box polypeptide 4; deleted in azoospermia like; marker; stromal cell derived factor 1alpha; transition protein 2; unclassified drug, animal cell; animal experiment; animal model; Article; cell proliferation; controlled study; immunohistochemistry; male; male genital tract parameters; nonhuman; rat; real time polymerase chain reaction; sperm viability; spermatogenesis; spermatozoon count; testis injury; testis weight; Wistar rat
Page Range: pp. 142-148
Journal or Publication Title: Reproductive Toxicology
Volume: 73
Publisher: Elsevier Inc.
Identification Number: 10.1016/j.reprotox.2017.08.006
ISSN: 08906238
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/176

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