Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Systemic infection with Candida albicans in breast tumor bearing mice: Cytokines dysregulation and induction of regulatory T cells

(2019) Systemic infection with Candida albicans in breast tumor bearing mice: Cytokines dysregulation and induction of regulatory T cells. Journal de Mycologie Medicale.

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Objective: The effect of candidemia on immunologic parameters in breast tumor bearing patients is not well studied. Here, we hypothesised that candidemia in the tumor background may change the outcome of immunologic parameters and tumor condition. Method: Mice were divided into four groups, including normal, tumor, Candida infected (only Candidiasis) and tumor/Candidiasis groups. Tumor changes were recorded daily after tumor transplantation and induction of candidemia. Splenocytes of mice were harvested, cultured, and stimulated with PHA; afterwards, IL-4, IL-10, IFN-γ, TNF-α and TGF-β cytokines were assessed using ELISA kits. We also evaluated the population of CD4 + CD25 + Foxp3 + regulatory T cells in the tumor infiltrated and splenocytes. Results: The results showed that infection with C. albicans decreased the IFN-γ/IL-4 ratio in tumor/candidiasis and candidiasis groups versus their non-infected controls. IL-10, TGF-β and TNF-α levels increased in the candidiasis group. In addition, Candidemia led to an increase in the Treg population in tumor microenvironment and splenocytes of experimental groups compared with non-infected controls. Finally, candidemia increased tumor growth of tumor/Candidiasis group compared with the tumor group. Conclusion: It seems that systemic infection with C. albicans could not only induce regulatory T cells but also result in dysregulation of cytokine network and thereby facilitate tumor growth. © 2018 Elsevier Masson SAS

Item Type: Article
Keywords: CD4 antigen; cytokine; gamma interferon; interleukin 10; interleukin 2 receptor alpha; interleukin 4; phytohemagglutinin; transcription factor FOXP3; transforming growth factor alpha; transforming growth factor beta; tumor necrosis factor, animal cell; animal experiment; animal model; animal tissue; Article; breast tumor; cancer transplantation; Candida albicans; candidemia; candidiasis; CD4+ CD25+ T lymphocyte; cell culture; cell infiltration; controlled study; disease association; enzyme linked immunosorbent assay; female; immune response; immunological parameters; mouse; nonhuman; outcome assessment; protein analysis; regulatory mechanism; regulatory T lymphocyte; spleen; spleen cell; treatment response; tumor growth; tumor microenvironment
Page Range: pp. 49-55
Journal or Publication Title: Journal de Mycologie Medicale
Volume: 29
Number: 1
Identification Number: 10.1016/j.mycmed.2018.10.006
Depositing User: مهندس جمال محمودپور

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