Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Synergistic effects of arsenic trioxide and radiation: Triggering the intrinsic pathway of apoptosis

(2017) Synergistic effects of arsenic trioxide and radiation: Triggering the intrinsic pathway of apoptosis. Iranian Biomedical Journal.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study was to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Methods: Cells were treated by different concentrations of ATO either in presence or absence of IR. Viability and apoptosis pathway of both treated and control groups were evaluated using MTT assay and the expression analysis of Bax, Bcl-2, and caspase-3 genes, respectively. All treatments were performed on 100-μm diameter spheroids. Results: Results showed a significant reduction in the survival of the cells in all treated groups. As expected, cell survival was much less in combination treatment than treatment with only ATO. Moreover, combination therapy made Bax and caspase-3 up-regulated and Bcl-2 down-regulated. Conclusion: ATO and radiation had a synergistic apoptotic effect on GBM cells by up-regulation of caspase-3 and alteration of the Bax-Bcl-2 balance; therefore, ATO may act as a potential anti-cancer agent against GBM cells through triggering the mitochondrial pathway of apoptosis. © 2017, Pasteur Institute of Iran. All rights reserved.

Item Type: Article
Keywords: arsenic trioxide; caspase 3; protein Bax, apoptosis; Article; Bax gene; caspase 3 gene; cell membrane permeability; cell structure; cell survival; cell viability; controlled study; down regulation; drug efficacy; drug mechanism; gene; gene activation; gene expression regulation; glioblastoma cell line; human; human cell; in vitro study; ionizing radiation; MTT assay; proto oncogene; spheroid cell; upregulation
Page Range: pp. 330-337
Journal or Publication Title: Iranian Biomedical Journal
Volume: 21
Number: 5
Publisher: Pasteur Institute of Iran
Identification Number: 10.18869/acadpub.ibj.21.5.330
ISSN: 1028852X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/188

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