(2018) Effect of gestational age on migration ability of the human umbilical cord vein mesenchymal stem cells. Advances in Medical Sciences.
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Abstract
Purpose Migration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant factor in cell therapy. MSCs derived from different sources show different properties. Here we compared the migration ability of the term and the pre-term human umbilical cord vein MSCs (hUCV-MSCs). Materials/Methods MSCs were isolated from term and pre-term umbilical cord vein, and cultured to passage 3–4. Migration rate of both groups was assessed in the presence of 10 FBS using chemotaxis assay. Surface expression of CXCR4 was measured by flow cytometery. The relative gene expression of CXCR4, IGF1-R, PDGFRα MMP-2, MMP-9, MT1-MMP and TIMP-2 were evaluated using real time PCR. Results The isolation rate of the pre-term hUCV-MSCs was higher than the term hUCV-MSCs. Phenotype characteristics and differentiation ability of the term and pre-term hUCV-MSCs were not different. The migration rate of the pre-term hUCV-MSCs was more than the term hUCV-MSCs. Gene and surface expressions of the CXCR4 were both significantly higher in the pre-term hUCV-MSCs (P ≤ 0.05). The mRNA levels of PDGFRα MMP-2, MMP-9, MT1-MMP and TIMP-2 showed no significant difference between the two groups. Conclusion Our results showed that the gestational age can affect the migration ability of the hUCV-MSCs. © 2017 Medical University of Bialystok
Item Type: | Article |
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Keywords: | chemokine receptor CXCR4; gelatinase A; gelatinase B; matrix metalloproteinase 14; platelet derived growth factor alpha receptor; somatomedin receptor; tissue inhibitor of metalloproteinase 2; chemokine receptor CXCR4; CXCR4 protein, human, adipogenesis; Article; bone development; cell culture; cell differentiation; cell isolation; cell lineage; cell migration; chemotaxis assay; chondrogenesis; flow cytometry; gestational age; human; human cell; in vitro study; mesenchymal stem cell; phenotype; protein expression; surface property; umbilical vein; cell membrane; cell motion; cytology; gene expression regulation; mesenchymal stroma cell; metabolism; umbilical cord; umbilical vein, Cell Differentiation; Cell Membrane; Cell Movement; Gene Expression Regulation; Gestational Age; Humans; Mesenchymal Stromal Cells; Receptors, CXCR4; Umbilical Cord; Umbilical Veins |
Page Range: | pp. 119-126 |
Journal or Publication Title: | Advances in Medical Sciences |
Volume: | 63 |
Number: | 1 |
Identification Number: | 10.1016/j.advms.2017.08.002 |
Depositing User: | مهندس جمال محمودپور |
URI: | http://eprints.muk.ac.ir/id/eprint/1886 |
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