Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

The potential role of humans in the transmission cycle of Leishmania major (Kinetoplastida: Trypanosomatidae), the causative agent of the Old World Zoonotic Cutaneous Leishmaniasis

(2018) The potential role of humans in the transmission cycle of Leishmania major (Kinetoplastida: Trypanosomatidae), the causative agent of the Old World Zoonotic Cutaneous Leishmaniasis. Journal of Medical Entomology.

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major (Yakimoff and Schokhor, 1914) (Kinetoplastida: Trypanosomatidae) is known as a neglected tropical disease, transmitted by Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae) in North Africa and the Middle East. The main reservoirs of ZCL are desert gerbils and the role of humans as a reservoir host of the disease is not clearly defined and is therefore investigated in this study. In order to achieve this objective, Ph. papatasi sand flies were allowed to take blood through indirect blood sources (human, Rhombomys opimus (Lichtenstein, 1823) (Rodentia: cricetidae) (great gerbil), and BALB/c mice) using artificial feeding or direct xenodiagnoses from ZCL patients. To detect Leishmania promastigotes inside digestive canal, blood-fed sand flies were dissected and examined under light microscope and then confirmed by nested-PCR. In indirect xenodiagnoses, promastigote forms of Leishmania parasite were observed in 7.1, 12, and 13.6 of sand flies which fed on Rh. opimus, BALB/c mice, or human blood sources, respectively. In direct xenodiagnoses, among 93 female lab-reared sand flies which fed on active L. major lesion(s), 2.1 Leishmania infection was subsequently observed in the sand flies. No infection was detected in those sand flies that fed on nonlesioned skin of CL patients. Humans can serve as a reservoir of ZCL since the data indicate that Ph. papatasi is able to acquire L. major parasites from an active lesion of ZCL patients and the parasites can complete metacyclogenesis in the sand fly. © The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.

Item Type: Article
Keywords: Artificial feeding; Human reservoir; Leishmania; Phlebotomus; Zoonotic cutaneous leishmaniasis
Page Range: pp. 1588-1593
Journal or Publication Title: Journal of Medical Entomology
Volume: 55
Number: 6
Identification Number: 10.1093/jme/tjy110
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/1924

Actions (login required)

View Item View Item