Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Evaluation of sperm chromatin integrity using aniline blue and toluidine blue staining in infertile and normozoospermic men

(2019) Evaluation of sperm chromatin integrity using aniline blue and toluidine blue staining in infertile and normozoospermic men. Journal of Reproduction and Infertility.

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Official URL: https://www2.scopus.com/inward/record.uri?eid=2-s2...

Abstract

Background: Male infertility is defined as a man lost his ability to fertilize a fertile female naturally. Diagnosis of male infertility cannot be made just according to basic semen analysis. It is necessity to have specific tests for evaluation of chromatin integrity. In this study, an attempt was made to evaluate the sperm chromatin quality in fertile men and infertile subgroup. Methods: Among 1386 couples, 342 men were categorized into normospermia and 1044 were infertile and they were referred to Yazd Research and Clinical Center for infertility treatment. Standard semen analysis and sperm nuclear maturity tests including aniline blue (AB) and toluidine blue (TB) staining were done. Data were analyzed by SPSS software. The p=0.05 was considered statistically significant. Results: The mean value of TB staining was significantly higher in infertile group compared to normospermic group (p=0.005). Mean of sperm normal morphology was lower in idiopathic infertile men in comparison with normozoospermic men (p= 0.001). The highest negative correlation was obtained between sperm count and AB staining. Progressive motility was negatively correlated with AB and TB staining in both groups but there was no significant difference between AB staining and progressive motility in men normospermia group. Conclusion: Sperm chromatin staining using AB and TB showed a negative association between sperm chromatin condensation with sperm count, normal morphology and progressive motility. It seems that the AB and TB test may be useful for the assessment of male fertility potential. © 2019 Avicenna Research Institute. All rights reserved.

Item Type: Article
Keywords: aniline blue; dye; tolonium chloride; unclassified drug, adult; aniline blue staining; Article; asthenospermia; chromatin; chromatin condensation; clinical evaluation; comparative study; controlled study; correlation coefficient; diagnostic test accuracy study; human; idiopathic disease; major clinical study; male; male fertility; male infertility; morphology; oligospermia; retrospective study; semen analysis; sensitivity and specificity; sperm quality; spermatozoon count; spermatozoon density; spermatozoon maturation; spermatozoon motility; staining; toluidine blue staining
Page Range: pp. 95-101
Journal or Publication Title: Journal of Reproduction and Infertility
Volume: 20
Number: 2
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/1976

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