Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Impedimetric mechanism study of horseradish peroxidase at low and high concentrations of hydrogen peroxide based on graphene/sol-gel/horseradish peroxidase

(2019) Impedimetric mechanism study of horseradish peroxidase at low and high concentrations of hydrogen peroxide based on graphene/sol-gel/horseradish peroxidase. International Journal of Biological Macromolecules.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

A novel electrochemical modified electrode for the impedimetric mechanism study of horseradish peroxidase and hydrogen peroxide was proposed based on immobilizing horseradish peroxidase/3‑(trimethoxysilyl) propylamine/graphene on glassy carbon electrode (HRP/TMSPA/Gr/GCE). Cyclic voltammetry and SEM were utilized to corroborate the successful stepwise assemblage procedure of the electrode. The electrochemical impedance spectroscopy measurements revealed that the charge transfer resistance increases at low concentrations and significantly decreases at high concentrations after enzymatic reaction with hydrogen peroxide. The mechanism study of the horseradish peroxidase and hydrogen peroxide were also investigated by electrochemical impedance spectroscopy. © 2018 Elsevier B.V.

Item Type: Article
Keywords: 3 (trimethoxysilyl)propylamine; graphene; horseradish peroxidase; hydrogen peroxide; propylamine; silica gel; unclassified drug; carbon nanotube; graphite; horseradish peroxidase; hydrogen peroxide; immobilized enzyme, Article; concentration (parameters); controlled study; cyclic potentiometry; glassy carbon electrode; impedance spectroscopy; impedimetric biosensor; room temperature; scanning electron microscopy; chemistry; electrochemistry; electrode; phase transition, Dielectric Spectroscopy; Electrochemistry; Electrodes; Enzymes, Immobilized; Graphite; Horseradish Peroxidase; Hydrogen Peroxide; Nanotubes, Carbon; Phase Transition
Page Range: pp. 677-681
Journal or Publication Title: International Journal of Biological Macromolecules
Volume: 123
Identification Number: 10.1016/j.ijbiomac.2018.11.091
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/2092

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