Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Fabrication of a glycation induced amyloid nanofibril and polyalizarin yellow R nanobiocomposite: Application for electrocatalytic determination of hydrogen peroxide

(2019) Fabrication of a glycation induced amyloid nanofibril and polyalizarin yellow R nanobiocomposite: Application for electrocatalytic determination of hydrogen peroxide. International Journal of Biological Macromolecules.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE). The electrocatalytic response of the biosensor was proportional to the hydrogen peroxide concentration in the range of 1 μM to 2.2 mM with a limit of detection and sensitivity of 290 nM and 0.024 μA/μM, respectively. The modified electrode demonstrated many advantages such as high sensitivity, low detection of limit and excellent catalytic activity. © 2018 Elsevier B.V.

Item Type: Article
Keywords: alizarin; amyloid; bovine serum albumin; buffer; fructose; hydrogen peroxide; nanocomposite; nanofiber; polyalizarin yellow R; thioflavine; unclassified drug; alizarin yellow R; amyloid; azo compound; hydrogen peroxide; nanocomposite; nanofiber, Article; catalysis; concentration (parameters); controlled study; electrochemistry; fluorescence analysis; glycation; incubation time; limit of detection; micromorphology; molecular size; nanofabrication; photon correlation spectroscopy; sensitivity analysis; transmission electron microscopy; animal; bovine; chemistry; electrode; fluorescence; genetic procedures; glycosylation; impedance spectroscopy; milk; oxidation reduction reaction; procedures; reproducibility; spectrofluorometry; ultrastructure, Amyloid; Animals; Azo Compounds; Biosensing Techniques; Catalysis; Cattle; Dielectric Spectroscopy; Dynamic Light Scattering; Electrochemistry; Electrodes; Fluorescence; Glycosylation; Hydrogen Peroxide; Milk; Nanocomposites; Nanofibers; Oxidation-Reduction; Reproducibility of Results; Serum Albumin, Bovine; Spectrometry, Fluorescence
Page Range: pp. 1297-1304
Journal or Publication Title: International Journal of Biological Macromolecules
Volume: 123
Publisher: Elsevier B.V.
Identification Number: 10.1016/j.ijbiomac.2018.10.043
ISSN: 01418130
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/2093

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