Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Albumin binding and anticancer effect of magnesium oxide nanoparticles

(2019) Albumin binding and anticancer effect of magnesium oxide nanoparticles. International Journal of Nanomedicine.

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Background: Recently, nanomaterials have moved into biological and medicinal implementations like cancer therapy. Therefore, before clinical trials, their binding to plasma proteins like human serum albumin (HSA) and their cytotoxic effects against normal and cancer cell lines should be addressed. Methods: Herein, the interaction of magnesium oxide nanoparticles (MgO NPs) with HSA was studied by means of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and docking studies. Afterwards, the cytotoxic impacts of MgO NPs on human leukemia cell line (K562) and peripheral blood mononucleated cells (PBMCs) were evaluated by MTT and flow cytometry assays to quantify reactive oxygen species (ROS) generation and apoptosis. Results: It was demonstrated that MgO NPs spontaneously form a static complex with HSA molecules through hydrophobic interactions. Docking study based on the size of NPs demonstrated that different linkages can be established between MgO NPs and HSA. The CD investigation explored that MgO NPs did not alter the secondary structure of HSA. Cellular studies revealed that MgO NPs induced cytotoxicity against K562 cell lines, whereas no adverse effects were detected on PBMCs up to optimum applied concentration of MgO NPs. It was exhibited that ROS production mediated by IC50 concentrations of MgO NPs caused apoptosis-associated cell death. The pre-incubation of K562 with ROS scavenger (curcumin) inhibited the impact of MgO NPs-based apoptosis on cell fate, revealing the upstream effect of ROS in our system. Conclusion: In summary, MgO NPs may exhibit strong plasma distribution and mediate apoptosis by ROS induction in the cancer cell lines. These data demonstrate a safe aspect of MgO NPs on the proteins and normal cells and their application as a distinctive therapeutic approach in the cancer treatment. © 2019 Behzadi et al.

Item Type: Article
Keywords: albumin; antineoplastic agent; magnesium oxide nanoparticle; nanoparticle; reactive oxygen metabolite; unclassified drug; human serum albumin; magnesium oxide; metal nanoparticle, antineoplastic activity; apoptosis; Article; binding affinity; binding site; cancer cell destruction; cell fate; circular dichroism; concentration response; controlled study; cytotoxicity test; drug identification; drug mechanism; drug protein binding; flow cytometry; human; human cell; hydrophobicity; in vitro study; K-562 cell line; molecular docking; MTT assay; particle size; peripheral blood mononuclear cell; spectrofluorometry; zeta potential; cell proliferation; chemistry; chronic myeloid leukemia; drug effect; metabolism; pathology; protein conformation; tumor cell culture, Cell Proliferation; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Magnesium Oxide; Metal Nanoparticles; Molecular Docking Simulation; Protein Conformation; Serum Albumin, Human; Tumor Cells, Cultured
Page Range: pp. 257-270
Journal or Publication Title: International Journal of Nanomedicine
Volume: 14
Identification Number: 10.2147/IJN.S186428
Depositing User: مهندس جمال محمودپور

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