Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: Biophysical, theoretical and cellular studies

(2019) The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: Biophysical, theoretical and cellular studies. International Journal of Nanomedicine.

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Aim: Nanoparticles (NPs) have been receiving potential interests in protein delivery and cell therapy. As a matter of fact, NPs may be used as great candidates in promoting cell therapy by catalase (CAT) delivery into high oxidative stress tissues. However, for using NPs like SiO2 as carriers, the interaction of NPs with proteins and mesenchymal stem cells (MSCs) should be explored in advance. Methods: In the present study, the interaction of SiO2 NPs with CAT and human MSCs (hMSCs) was explored by various spectroscopic methods (fluorescence, circular dichroism (CD), UV-visible), molecular docking and dynamics studies, and cellular (MTT, cellular morphology, cellular uptake, lactate dehydrogenase, ROS, caspase-3, flow cytometry) assays. Results: Fluorescence study displayed that both dynamic and static quenching mechanisms and hydrophobic interactions are involved in the spontaneous interaction of SiO2 NPs with CAT. CD spectra indicated that native structure of CAT remains stable after interaction with SiO2 NPs. UV-visible study also revealed that the kinetic parameters of CAT such as Km, Vmax, Kcat, and enzyme efficiency were not changed after the addition of SiO2 NPs. Molecular docking and dynamics studies showed that Si and SiO2 clusters interact with hydrophobic residues of CAT and SiO2 cluster causes minor changes in the CAT structure at a total simulation time of 200 ps. Cellular assays depicted that SiO2 NPs induce significant cell mortality, change in cellular morphology, cellular internalization, ROS elevation, and apoptosis in hMSCs at higher concentration than 100 µg/mL (170 µM). Conclusion: The current results suggest that low concentrations of SiO2 NPs induce no substantial change or mortality against CAT and hMSCs, and potentially useful carriers in CAT delivery to hMSC. © 2019 Mousavi et al. php and incorporate th.

Item Type: Article
Keywords: caspase 3; catalase; lactate dehydrogenase; nanocarrier; reactive oxygen metabolite; silica nanoparticle; silicon; silicon dioxide; caspase 3; catalase; lactate dehydrogenase; nanoparticle; reactive oxygen metabolite; silicon dioxide, apoptosis; Article; cell death; cell metabolism; cell structure; chemical reaction kinetics; circular dichroism; concentration (parameter); controlled study; cytotoxicity; drug delivery system; enzyme structure; flow cytometry; fluorescence analysis; human; human cell; hydrophobicity; internalization; kinetic parameters; mesenchymal stem cell; molecular docking; molecular dynamics; MTT assay; photochemical quenching; simulation; spectrofluorometry; static electricity; ultraviolet visible spectroscopy; biophysics; cell shape; chemistry; cytology; drug effect; endocytosis; kinetics; mesenchymal stem cell; metabolism; oxidative stress; theoretical model; thermodynamics, Apoptosis; Biophysical Phenomena; Caspase 3; Catalase; Cell Shape; Circular Dichroism; Endocytosis; Humans; Kinetics; L-Lactate Dehydrogenase; Mesenchymal Stem Cells; Models, Theoretical; Molecular Docking Simulation; Molecular Dynamics Simulation; Nanoparticles; Oxidative Stress; Reactive Oxygen Species; Silicon Dioxide; Spectrometry, Fluorescence; Thermodynamics
Page Range: pp. 5355-5368
Journal or Publication Title: International Journal of Nanomedicine
Volume: 14
Publisher: Dove Medical Press Ltd.
Identification Number: 10.2147/IJN.S210136
ISSN: 11769114
Depositing User: مهندس جمال محمودپور

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