Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Altered TH1, TH2, TH17 balance in assisted reproductive technology conceived mice

(2020) Altered TH1, TH2, TH17 balance in assisted reproductive technology conceived mice. Journal of Reproductive Immunology.

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Aim and purpose: The higher prevalence rate of different diseases may accentuate the possible alteration of the immune system in individuals conceived through the assisted reproductive technologies (ART). The present study was conducted to evaluate the TH1, TH2, TH17 balance in intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) - conceived mice in comparison to naturally conceived offspring. Methods: Mice (6–8 weeks) were divided into three groups (IVF- conceived, ICSI- conceived and naturally conceived). They were subjected to subcutaneous immunization witMycobacterium bovis Bacille Calmette-Guérin (BCG). The blood samples were taken and the sera were separated. Then the spleens were surgically removed at the time the mice were sacrificed. Serum levels of IFN-γ, IL-17A and IL-4 were detected by ELISA. Then the proportion of TCD4 cells possessing the T-bet+, GATA3+, and ROR-γt + were measured using FACS caliber flow cytometer. Results: In comparison with naturally conceived mice, intracellular expression of T-bet and serum levels of IFN-γ were significantly decreased in ART- conceived mice. Moreover, ART- conceived offspring showed marked increase in IL-4 and IL-17A. Conclusion: It is concluded that compared to naturally conceived male mice, ART- conceived counterparts exhibit less efficient immune responses against BCG through further promotion of humoral and inflammatory related immune response characteristics. © 2020

Item Type: Article
Keywords: gamma interferon; interleukin 17; interleukin 4; protein; ror gamma t protein; transcription factor GATA 3; transcription factor T bet; unclassified drug, Article; blood sampling; enzyme linked immunosorbent assay; female; flow cytometry; fluorescence activated cell sorting; in vitro fertilization; intracytoplasmic sperm injection; male; mouse; nonhuman; priority journal; progeny; protein blood level; Th1 cell; Th17 cell; Th2 cell
Journal or Publication Title: Journal of Reproductive Immunology
Volume: 139
Identification Number: 10.1016/j.jri.2020.103117
Depositing User: مهندس جمال محمودپور

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