Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Expression of Helicobacter pylori CagL gene in Lactococcus lactis MG1363 and evaluation of its immunogenicity as an oral vaccine in mice

(2020) Expression of Helicobacter pylori CagL gene in Lactococcus lactis MG1363 and evaluation of its immunogenicity as an oral vaccine in mice. Microbial Pathogenesis.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Helicobacter pylori is a gram negative pathogen which commonly colonizes in the human gastric mucosa from early childhood and persists throughout life. CagL is a 27-kDa protein that is located at the tip of T4SS pili and highly conserved among pathogenic H. pylori strains. Lactic acid bacteria especially Lactococcus lactis (L. lactis) could serve as an antigen-delivering vehicle for the development of edible vaccine. In this study H. pylori CagL gene was cloned in pAMJ2008 vector and transferred to Lactococcus lactis MG1363 as the host for CagL antigen production. This recombinant bacterium was orally subjected to mice, and the immune response to CagL was evaluated by ELISA. Intracellular expression of CagL protein was confirmed by Western blot analysis. Mucosal immunization of mice with the recombinant L. lactis significantly stimulated CagL-Specific antibodies: IgA, IgG, cytokine IL-17 and IN-γ. Moreover, the specific anti-CagL IgA response was detected in the feces of immunized mice. These results indicate that CagL of H. pylori was successfully expressed in L. lactis and the recombinant bacteria can be potentially used as an edible vaccine against H. pylori infection. © 2019

Item Type: Article
Keywords: bacterial protein; bacterial vaccine; CagL protein; gamma interferon; immunoglobulin A antibody; immunoglobulin G antibody; interleukin 17; unclassified drug, animal experiment; animal model; antibody response; antibody titer; Article; bacterial gene; CagL gene; controlled study; enzyme linked immunosorbent assay; evaluation study; gene expression; gene sequence; Helicobacter infection; Helicobacter pylori; immune response; immunization; immunogenicity; Lactococcus lactis; male; mouse; nonhuman; priority journal; protein expression; protein purification; Western blotting
Journal or Publication Title: Microbial Pathogenesis
Volume: 142
Identification Number: 10.1016/j.micpath.2019.103926
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/2186

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