Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Isolation and identification of indigenous prokaryotic bacteria from arsenic-contaminated water resources and their impact on arsenic transformation

(2017) Isolation and identification of indigenous prokaryotic bacteria from arsenic-contaminated water resources and their impact on arsenic transformation. Ecotoxicology and Environmental Safety.

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Arsenic is a known human carcinogen. Arsenite As(III), H3AsO3 and arsenate As(V), H2AsO4 − and HAsO4 2- are the two predominant compounds of As found in surface water and groundwater. The aim of this study was to explore a bioremediation strategy for biotransformation of arsenite to arsenate by microorganisms. In this study, Babagorgor Spring, located west of Iran, was selected as the arsenic-contaminated source and its physicochemical characteristics and in situ microbiological composition were analyzed. Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) analysis indicated that the arsenic level was 614 μg/l. Fourteen arsenic tolerant indigenous bacteria were isolated from arsenic-contaminated water using chemically defined medium (CDM), supplemented with 260–3900 mg/l arsenite and 1560–21800 mg/l arsenate. Among the isolates, a strain As-11 exhibited high ability of arsenic transformation. Biochemical tests were used for bacterial identification and confirmation was conducted by 16 S rRNA sequence analysis. Results confirmed that As-11 was related to the genus Pseudomonas. This bacterium showed maximum tolerable concentration to arsenite up to 3250 mg/l and arsenate up to 20280 mg/l. Under heterotrophic conditions, the bacterium exhibited 48% of As(III) and 78% of As(V) transformation from the medium amended with 130 and 312 mg/l of sodium arsenite and sodium arsenate, respectively. Moreover, under chemolithotrophic conditions, bacterium was able to transform 41% of 130 mg/l of As(III) from the medium amended with nitrate as the terminal electron acceptor. Pseudomonas strain As-11 was reported as an arsenic transformer, for the first time. © 2017 Elsevier Inc.

Item Type: Article
Keywords: arsenic; arsenic trioxide; RNA 16S; arsenate sodium; arsenic; arsenic acid; arsenic acid derivative; arsenite sodium; arsenous acid derivative; ground water; RNA 16S; sodium derivative, arsenic; bacterium; bioremediation; concentration (composition); pollutant source; prokaryote; spring (hydrology); water pollution; water resource, Article; atomic emission spectrometry; bacterial strain; bacterium identification; bacterium isolation; bioremediation; biotransformation; chemical analysis; controlled study; heavy metal removal; Iran; microbial activity; nonhuman; physical chemistry; Pseudomonas; RNA sequence; sequence analysis; water contamination; water supply; adaptation; analysis; biotransformation; genetics; metabolism; microbiology; physiology; water pollution, Iran, Bacteria (microorganisms); Prokaryota; Pseudomonas, Adaptation, Physiological; Arsenates; Arsenic; Arsenites; Biodegradation, Environmental; Biotransformation; Groundwater; Iran; Pseudomonas; RNA, Ribosomal, 16S; Sodium Compounds; Water Pollution; Water Resources
Page Range: pp. 170-176
Journal or Publication Title: Ecotoxicology and Environmental Safety
Volume: 140
Publisher: Academic Press
Identification Number: 10.1016/j.ecoenv.2017.02.051
ISSN: 01476513
Depositing User: مهندس مهدی شریفی

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