Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction

(2017) Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction. Revista da Sociedade Brasileira de Medicina Tropical.

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Abstract

Introduction: Infections caused by β-lactamase-producing gram-negative bacteria, such as Klebsiella pneumoniae, are increasing globally with high morbidity and mortality. The aim of the current study was to determine antimicrobial susceptibility patterns and the prevalence of antibiotic resistance genes (β-lactamase and integron genes) using multiplex PCR. Methods: One-hundred K. pneumoniae isolates were collected from different clinical samples. Antibiotic susceptibility testing was performed with thirteen different antibiotics. Multiplex-PCR was used to detect β-lactamase (blaTEM, blaCTX-M, blaSHV, blaVEB, blaPER, blaGES, blaVIM, blaIMP, blaOXA, and blaKPC) and integron genes (int I, int II, and int III). Results: The highest and lowest rate of resistance was exhibited against amikacin (93) and imipenem (8), respectively. The frequency of β-lactamase-positive K. pneumoniae was 37, and the prevalence of the blaTEM, blaCTX-M, blaSHV, blaVEB, blaPER, blaGES, blaVIM, blaIMP, blaOXA, and blaKPC genes was 38, 24, 19, 12, 6, 11, 33, 0, 28, and 23, respectively. Of the 100 isolates, eight (8) were positive for class I integrons; however, class II and III integrons were not detected in any of the strains. Conclusions: These results indicate co-carriage of a number of β-lactamase genes and antibiotic resistance integrons on the same plasmids harboring multi-drug resistance genes. It seems that these properties help to decrease treatment complications due to resistant bacterial infections by rapid detection, infection-control programs and prevention of transmission of drug resistance. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.

Item Type: Article
Keywords: amikacin; amoxicillin plus clavulanic acid; ampicillin; aztreonam; beta lactamase; cefepime; cefotaxime; ceftazidime; ceftriaxone; ciprofloxacin; cotrimoxazole; gentamicin; imipenem; levofloxacin; antiinfective agent; bacterial DNA; beta lactamase, adolescent; adult; aged; antibiotic sensitivity; Article; bacterial gene; bacterium isolate; beta lactamase gene; child; cross-sectional study; female; gene amplification; human; integron; integron gene; Klebsiella pneumoniae; major clinical study; male; multiplex polymerase chain reaction; nonhuman; quality control; antibiotic resistance; drug effects; genetics; integron; isolation and purification; Klebsiella infection; Klebsiella pneumoniae; microbial sensitivity test; microbiology; multiplex polymerase chain reaction, Anti-Bacterial Agents; beta-Lactamases; Cross-Sectional Studies; DNA, Bacterial; Drug Resistance, Bacterial; Humans; Integrons; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Multiplex Polymerase Chain Reaction
Page Range: pp. 321-328
Journal or Publication Title: Revista da Sociedade Brasileira de Medicina Tropical
Volume: 50
Number: 3
Publisher: Sociedade Brasileira de Medicina Tropical
Identification Number: 10.1590/0037-8682-0001-2017
ISSN: 00378682
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/258

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