Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Evaluation of intracellular anion superoxide level, heat shock protein a2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

(2017) Evaluation of intracellular anion superoxide level, heat shock protein a2 and protamine positive spermatozoa percentages in teratoasthenozoospermia. International Journal of Reproductive BioMedicine.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology.Objective: To compare the level of intracellular anion superoxide (O2–), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men. Materials and Methods: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4 and total motility lower than 40) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2 – and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test. Results: The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2 – levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2 – and HSPA2 in the case group (r=0.041, p=0.79). Conclusion: We suggest that the increase in intracellular O2 –, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients. © 2017, Research and Clinical Center for Infertitlity. All rights reserved.

Item Type: Article
Keywords: chromomycin A3; heat shock protein; protamine; superoxide, adult; apoptosis; Article; asthenospermia; case control study; cellular distribution; clinical article; controlled study; flow cytometry; fluorescence activated cell sorting; human; male; male infertility; protein deficiency; semen analysis; spermatozoon; spermatozoon count; spermatozoon motility; teratogenesis
Page Range: pp. 279-286
Journal or Publication Title: International Journal of Reproductive BioMedicine
Volume: 15
Number: 5
Publisher: Research and Clinical Center for Infertitlity
ISSN: 24764108
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/259

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