Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Altered helper T cell-mediated immune responses in male mice conceived through in vitro fertilization

(2017) Altered helper T cell-mediated immune responses in male mice conceived through in vitro fertilization. Reproductive Toxicology.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

A study using a mouse IVF model was conducted to examine the hypothesis that in vitro fertilization (IVF) treatment may lead to immune system alteration in the offspring. Phagocytic activity and lymphocyte proliferative responses to mitogen, alloantigen, and purified protein derivative (PPD) of Mycobacterium bovis were investigated in the splenocytes of BCG-treated male mice conceived by IVF or natural conception. Intracellular expression of T-bet and GATA3 in helper T-cell population were examined in both groups. Moreover, the serum levels of IFN-γ and IL-4 along with BCG-specific levels of IgG1 and IgG2a were assessed by ELISA. In comparison with naturally-conceived mice, PPD-specific proliferative response and T-bet/GATA3 ratio were significantly decreased in IVF-conceived mice. Moreover, IVF-conceived mice exhibited marked decreases in IFN-γ/IL-4 and IgG2a/IgG1 ratios. Results indicate that in comparison with male mice conceived by natural conception, IVF counterparts exhibit less efficient immune responses against BCG through further promotion of Th2 responses. © 2017 Elsevier Inc.

Item Type: Article
Keywords: alloantigen; BCG vaccine; gamma interferon; immunoglobulin G1; immunoglobulin G2a; interleukin 4; mitogenic agent; transcription factor GATA 3; transcription factor T bet; tuberculin, animal cell; animal experiment; Article; conception; controlled study; enzyme linked immunosorbent assay; female; helper cell; immune response; in vitro fertilization; lymphocyte proliferation; lymphocyte subpopulation; male; mouse; Mycobacterium bovis; nonhuman; phagocytosis; progeny; protein blood level; protein expression; spleen cell
Page Range: pp. 196-203
Journal or Publication Title: Reproductive Toxicology
Volume: 69
Publisher: Elsevier Inc.
Identification Number: 10.1016/j.reprotox.2017.03.005
ISSN: 08906238
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/272

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