Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients

(2017) Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients. Archives of Gynecology and Obstetrics.

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Purpose: AKTs have a pivotal role in the granulosa-lutein cell (GC) proliferation and folliculogenesis, and there is a reciprocal feedback between AKT with androgen. Therefore, we aimed to evaluate the role of AKTs in GCs of hyperandrogenic (+HA) PCOS cases. Method: There were three groups: control, +HA PCOS and –HA (non-hyperandrogenic) PCOS. All groups were subjected to GnRH antagonist protocol for stimulation of ovulation. Follicular fluid was aspirated from large follicles, and GCs were isolated using cell strainer method. AKT1, AKT2, AKT3, and androgen receptor (AR) mRNA expressions were analyzed with quantitative real-time PCR (qRT-PCR), and total-AKT and p-AKT (Ser473 & Thr308) were investigated using western blotting. Results: There were high levels of AKT1, AKT2, and AR mRNA expressions and high levels of p-AKT protein expression in the +HA PCOS group (p ≤ 0.05). There was a direct positive correlation between free testosterone (FT) and total testosterone (TT) with the levels of AKT1, AKT2, and p-AKT (Ser473), and also between FT with the levels of AR. Conclusion: High expressions of AKT1 and AKT2 through possible relation with androgen may cause GCs dysfunction in the +HA PCOS patients. © 2017, Springer-Verlag Berlin Heidelberg.

Item Type: Article
Keywords: androgen receptor; messenger RNA; prasterone sulfate; sex hormone binding globulin; testosterone; AKT1 protein, human; AKT2 protein, human; protein kinase B; testosterone, adult; AKT1 gene; AKT2 gene; Article; body mass; cell isolation; cell proliferation; cell survival; cell viability; clinical protocol; controlled study; correlation coefficient; female; flow cytometry; granulosa cell; human; linear regression analysis; luteal cell; major clinical study; ovary follicle development; ovary polycystic disease; ovulation; protein phosphorylation; quantitative analysis; real time polymerase chain reaction; sequence homology; Western blotting; young adult; blood; complication; genetics; granulosa cell; hyperandrogenism; luteal cell; metabolism; ovary follicle fluid; ovary polycystic disease; ovulation induction; pathophysiology; statistical model, Adult; Female; Follicular Fluid; Granulosa Cells; Humans; Hyperandrogenism; Linear Models; Luteal Cells; Ovulation Induction; Polycystic Ovary Syndrome; Proto-Oncogene Proteins c-akt; Real-Time Polymerase Chain Reaction; Testosterone
Page Range: pp. 1041-1050
Journal or Publication Title: Archives of Gynecology and Obstetrics
Volume: 295
Number: 4
Publisher: Springer Verlag
Identification Number: 10.1007/s00404-017-4317-9
ISSN: 09320067
Depositing User: مهندس مهدی شریفی

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