Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

The combined application of human adipose derived stem cells and Chondroitinase ABC in treatment of a spinal cord injury model

(2017) The combined application of human adipose derived stem cells and Chondroitinase ABC in treatment of a spinal cord injury model. Neuropeptides.

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Background Although stem cell therapy has become a major focus as a new option for management of spinal cord injury (SCI), its effectiveness should be promoted. In this study, we investigated the effects of co-administrating human adipose-derived stem cells (hADSCs) and Chondroitinase ABC (ChABC) in a rat model of spinal cord injury. Material and methods hADSCs derived from superficial layer of abdominal adipose tissue were used to treat a contusion-induced SCI. Animals were randomly allocated to five equal groups including sham (only laminectomy), SCI (SCI + vehicle injection), hADSCs (1 × 10⁶ hADSCs/10 μl intra-spinal injection), ChABC (10 μl of 100 U/ml ChABC intra-spinal injection injection), and hADSCs + ChABC. Basso, Beattie and Bresnahan tests were used to evaluate locomotor function. 8 weeks after treatment, cavity size, myelination, cell differentiation (neuron and astrocyte), and chondroitin sulfate amount were analyzed. Results hADSC transplanted animals, ChABC injected animals (P < 0.001), and hADSC + ChABC treated rats (P < 0.001) displayed significant motor improvement compared to SCI group. Combination therapy of hADSCs and ChABC led to greater locomotor recovery compared to using hADSCs (P < 0.001) or ChABC (P < 0.01) alone. Spinal cords in the combined and single therapy groups had cavities filled with myelinated areas and less chondroitin sulfate content in comparison with the control group (P < 0.001). hADSCs expressed GFAP, B III tubulin and Map2. Conclusion Combination therapy with ChABC and hADSCs exhibits more significant functional recovery than single therapy using either. This result may be applicable in selection of the best therapeutic strategy for SCI. © 2016 Elsevier Ltd

Item Type: Article
Keywords: 5' nucleotidase; b iii tubulin; beta tubulin; chondroitin ABC lyase; chondroitin sulfate; glial fibrillary acidic protein; Hermes antigen; microtubule associated protein 2; myelin; Thy 1 antigen; unclassified drug; chondroitin ABC lyase, adipose derived stem cell; adult; animal experiment; animal model; animal tissue; Article; astrocyte; basso beattie and bresnahan test; cell aggregation; cell differentiation; cell structure; cell survival; controlled study; flow cytometry; human; human cell; immunohistochemistry; locomotion; male; mesenchyme cell; motor dysfunction assessment; myelination; nerve cell; nonhuman; priority journal; rat; spinal cord injury; spindle cell; stem cell transplantation; animal; convalescence; disease model; drug effects; laminectomy; multimodality cancer therapy; nerve regeneration; pathophysiology; Spinal Cord Injuries; treatment outcome; Wistar rat, Animals; Cell Differentiation; Chondroitin ABC Lyase; Combined Modality Therapy; Disease Models, Animal; Humans; Laminectomy; Male; Nerve Regeneration; Neurons; Rats; Rats, Wistar; Recovery of Function; Spinal Cord Injuries; Stem Cell Transplantation; Treatment Outcome
Page Range: pp. 39-47
Journal or Publication Title: Neuropeptides
Volume: 61
Publisher: Churchill Livingstone
Identification Number: 10.1016/j.npep.2016.07.004
ISSN: 01434179
Depositing User: مهندس مهدی شریفی

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