Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

The leishmanicidal effect of Lucilia sericata larval saliva and hemolymph on in vitro Leishmania tropica

(2021) The leishmanicidal effect of Lucilia sericata larval saliva and hemolymph on in vitro Leishmania tropica. Parasites and Vectors.

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Abstract

Background: Leishmaniasis is a major parasitic disease worldwide, except in Australia and Antarctica, and it poses a signifcant public health problem. Due to the absence of safe and efective vaccines and drugs, researchers have begun an extensive search for new drugs. The aim of the current study was to investigate the in vitro leishmanicidal activity of larval saliva and hemolymph of Lucilia sericata on Leishmania tropica. Methods: The efects of diferent concentrations of larval products on promastigotes and intracellular amastigotes of L. tropica were investigated using the mouse cell line J774A.1 and peritoneal macrophages as host cells. The 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and direct observation and counting method were used to assess the inhibitory efects and cell cytotoxicity of the larval products. The efects of larval products on the amastigote form of L. tropica were quantitatively estimated by calculating the rate of macrophage infection, number of amastigotes per infected macrophage cell, parasite load and survival index. Results: The 50% cytotoxicity concentration (CC50) value of both larval saliva and hemolymph was 750 µg/ml, and the 50% inhibitory concentration (IC50) values were 134 µg/ml and 60 µg/ml for larval saliva and larval hemolymph, respectively. The IC50 for Glucantime, used a positive control, was (11.65 µg/ml). Statistically signifcant diferences in viability percentages of promastigotes were observed for diferent doses of both larval saliva and hemolymph when compared with the negative control (p ≤ 0.0001). Microscopic evaluation of the amastigote forms revealed that

Item Type: Article
Keywords: Anti-leishmanial activity, Saliva, Leishmania tropica, Promastigote, Intracellular amastigote, Hemolymph
Subjects: WC Communicable Diseases > WC 680-950 Tropical and Parasitic Diseases
Divisions: Research Vice-Chancellor Department > Zoonoses Research Center
Journal or Publication Title: Parasites and Vectors
Journal Index: ISI
Volume: 14
Number: 1
Publisher: BioMed Central Ltd
Identification Number: 10.1186/s13071-020-04543-y
ISSN: 17563305
Depositing User: مسعود رسول آبادی
URI: http://eprints.muk.ac.ir/id/eprint/4208

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