Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

rRT-PCR for SARS-CoV-2: Analytical considerations

(2021) rRT-PCR for SARS-CoV-2: Analytical considerations. Clinica Chimica Acta.

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he COVID-19 pandemic remains a significant problem involving health systems worldwide. Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard. Although this test has high specificity and relatively high sensitivity, the occurrence of falsely negative results in symptomatic patients and/or having a positive CT scan remains a challenge. Sources of error can be pre-analytical (sampling, storage and processing), analytical (RNA extraction, cDNA synthesis and amplification) and post-analytical (interpretation and analysis and test reporting). These potential sources of error and efforts to mitigate are reviewed in this article with an emphasis on the analytical phase. © 2021 Elsevier B.V

Item Type: Article
Keywords: COVID-19; Laboratory errors; rRT-PCR method; SARS-CoV-2
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry
Page Range: pp. 1-7
Journal or Publication Title: Clinica Chimica Acta
Journal Index: ISI
Volume: 516
Publisher: Elsevier B.V.
Identification Number: 10.1016/j.cca.2021.01.011
ISSN: 00098981
Depositing User: مسعود رسول آبادی

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