(2021) rRT-PCR for SARS-CoV-2: Analytical considerations. Clinica Chimica Acta.
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Abstract
he COVID-19 pandemic remains a significant problem involving health systems worldwide. Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard. Although this test has high specificity and relatively high sensitivity, the occurrence of falsely negative results in symptomatic patients and/or having a positive CT scan remains a challenge. Sources of error can be pre-analytical (sampling, storage and processing), analytical (RNA extraction, cDNA synthesis and amplification) and post-analytical (interpretation and analysis and test reporting). These potential sources of error and efforts to mitigate are reviewed in this article with an emphasis on the analytical phase. © 2021 Elsevier B.V
Item Type: | Article |
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Keywords: | COVID-19; Laboratory errors; rRT-PCR method; SARS-CoV-2 |
Subjects: | QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body |
Divisions: | Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry |
Page Range: | pp. 1-7 |
Journal or Publication Title: | Clinica Chimica Acta |
Journal Index: | ISI |
Volume: | 516 |
Publisher: | Elsevier B.V. |
Identification Number: | 10.1016/j.cca.2021.01.011 |
ISSN: | 00098981 |
Depositing User: | مسعود رسول آبادی |
URI: | http://eprints.muk.ac.ir/id/eprint/4213 |
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