Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Biodegradation of 2,4-dichlorophenoxyacetic acid by bacteria with highly antibiotic-resistant pattern isolated from wheat field soils in Kurdistan, Iran

(2016) Biodegradation of 2,4-dichlorophenoxyacetic acid by bacteria with highly antibiotic-resistant pattern isolated from wheat field soils in Kurdistan, Iran. Environmental Monitoring and Assessment.

Full text not available from this repository.

Official URL:


Recently, there has been increasing interest to clean up the soils contaminated with herbicide. Our aim was to determine the bioremediation of 2,4-dichlorophenoxyacetic acid (2,4-D) from wheat fields which have a long history of herbicide in Sanandaj. Based on our literature survey, this study is the first report to isolate and identify antimicrobial resistant bacteria from polluted wheat field soils in Sanandaj which has the capacity to degrade 2,4-D. From 150 2,4-D-exposed soil samples, five different bacteria were isolated and identified based on biochemical tests and 16S ribosomal RNA (rRNA). Pseudomonas has been the most frequently isolated genus. By sequencing the 16S rRNA gene of the isolated bacteria, the strains were detected and identified as a member of the genus Pseudomonas sp, Entrobacter sp, Bacillus sp, Seratia sp, and Staphylococcus sp. The sequence of Sanandaj 1 isolate displayed 87 similarity with the 16S rRNA gene of a Pseudomonas sp (HE995788). Similarly, all the isolates were compared to standard strains based on 16S rRNA. Small amounts of 2,4-D could be transmitted to a depth of 10–20 cm; however, in the depth of 20–40 cm, we could not detect the 2,4-D. The isolates were resistant to various antibiotics particularly, penicillin, ampicillin, and amoxicillin. © 2016, Springer International Publishing Switzerland.

Item Type: Article
Keywords: Antibiotics; Bacteria; Bacteriology; Biodegradation; Bioremediation; Degradation; Genes; Herbicides; RNA; Soils; Weed control, 16S ribosomal RNA; 16S rRNA; 2 ,4-D degradation; 2 ,4-dichlorophenoxyacetic acid; 2 ,4-dichlorophenoxyacetic acid (2 ,4-D); Literature survey; Pseudomonas; Resistant bacteria, Soil pollution, 2,4 dichlorophenoxyacetic acid; amikacin; amoxicillin; ampicillin; gentamicin; nitrofurantoin; penicillin derivative; tetracycline; 2,4 dichlorophenoxyacetic acid; RNA 16S; soil pollutant, 2,4 dichlorophenoxyacetic acid; agricultural land; antibiotics; bacterium; biodegradation; bioremediation; herbicide; RNA, antibiotic resistance; Article; Bacillus; bacterial strain; bacterium; bacterium contamination; bacterium identification; bacterium isolation; biodegradation; chemical analysis; Enterobacter; gas chromatography; Iran; nonhuman; nucleotide sequence; physical chemistry; physical parameters; Pseudomonas; sequence analysis; Seratia; soil analysis; soil pollution; Staphylococcus; analysis; bioremediation; classification; environmental monitoring; genetics; growth, development and aging; isolation and purification; metabolism; microbiology; procedures; soil pollutant; wheat, Iran; Kordestan Iran; Sanandaj, Bacillus sp.; Pseudomonas; Pseudomonas sp.; Staphylococcus sp.; Triticum aestivum, 2,4-Dichlorophenoxyacetic Acid; Biodegradation, Environmental; Drug Resistance, Bacterial; Environmental Monitoring; Iran; Pseudomonas; RNA, Ribosomal, 16S; Soil Microbiology; Soil Pollutants; Triticum
Journal or Publication Title: Environmental Monitoring and Assessment
Volume: 188
Number: 12
Publisher: Springer International Publishing
Identification Number: 10.1007/s10661-016-5673-9
ISSN: 01676369
Depositing User: مهندس جمال محمودپور

Actions (login required)

View Item View Item