Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Efficacy of Crocus sativus L. on reduction of cadmium-induced toxicity on spermatogenesis in adult rats

(2016) Efficacy of Crocus sativus L. on reduction of cadmium-induced toxicity on spermatogenesis in adult rats. Andrologia.

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Cadmium is a toxic heavy metal element, which probably cause infertility by impairment in spermatogenesis. The present work aimed (i) to study the toxic effect of cadmium on spermatogenesis in rat, as well as (ii) the protective effect of Crocus sativus L. on cadmium-intoxicated rats. Cadmium chloride was administered intraperitoneally during 16 days at intervals of 48 h between subsequent treatments. Crocus sativus L. was pre-treated in both of control and cadmium-injected rats. Animals were sacrificed on day 17 after the first treatment. The left cauda epididymis was removed and immediately immersed into Hank's balanced salt solution for the evaluation of sperm count and viability, and left testis was fixed in 10 formalin for histological evaluation. Following contamination with cadmium, a decrease was observed in the number and viability of cauda epididymis sperm, which were increased by Crocus sativus L. pre-treatment (P < 0.05). In addition, cadmium decreased both cell proliferation and Johnsen Scores in the seminiferous tubules, which were reversed by Crocus sativus pre-treatment (P < 0.05). Furthermore, cadmium-induced decrease in the amount of free serum testosterone as well as an increase in lipid peroxidation activity in the testicular tissue was reversed by Crocus sativus L. (P < 0.05). These findings may support the concept that Crocus sativus L. can improve the cadmium toxicity on spermatogenesis. © 2016 Blackwell Verlag GmbH

Item Type: Article
Keywords: balanced salt solution; cadmium chloride; Crocus sativus extract; testosterone; thiobarbituric acid reactive substance; cadmium chloride; plant extract, adult; animal experiment; animal model; animal tissue; antioxidant activity; Article; cell proliferation; cell viability; controlled study; Crocus sativus; drug efficacy; epididymis; histology; immunohistochemistry; lipid peroxidation; male; nonhuman; oxidative stress; pistil stigma; protection; rat; reproductive toxicity; seminiferous tubule; spermatogenesis; spermatozoon count; testis; testosterone blood level; animal; Crocus; drug effects; spermatogenesis; spermatozoon; Wistar rat, Animals; Cadmium Chloride; Crocus; Epididymis; Lipid Peroxidation; Male; Plant Extracts; Rats; Rats, Wistar; Sperm Count; Spermatogenesis; Spermatozoa
Page Range: pp. 1244-1252
Journal or Publication Title: Andrologia
Volume: 48
Number: 10
Publisher: Blackwell Publishing Ltd
Identification Number: 10.1111/and.12568
ISSN: 03034569
Depositing User: مهندس جمال محمودپور

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