Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Extracellular vesicles derived from human umbilical cord perivascular cells improve functional recovery in brain ischemic rat via the inhibition of apoptosis brain ischemic rat via the inhibition of apoptosis

(2020) Extracellular vesicles derived from human umbilical cord perivascular cells improve functional recovery in brain ischemic rat via the inhibition of apoptosis brain ischemic rat via the inhibition of apoptosis. Iranian Biomedical Journal.

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Abstract

Background: Ischemic stroke, as a health problem caused by the reduced blood supply to the brain, can lead to the neuronal death. The number of reliable therapies for stroke is limited. MSCs exhibit therapeutic achievement. A major limitation of MSC application in cell therapy is the short survival span. MSCs affect target tissues through the secretion of many paracrine agents including EVs. This study aimed to investigate the effect of HUCPVCsderived EVs on apoptosis, functional recovery, and neuroprotection. Methods: Ischemia was induced by MCAO in male Wistar rats. Animals were classified into sham, MCAO, MCAO + HUCPVC, and MCAO + EV groups. Treatments began at two hours after ischemia. Expressions of apoptotic-related proteins (BAX/BCl-2 and caspase- 3 and -9), the amount of TUNEL-positive cells, neuronal density (MAP2), and dead neurons (Nissl staining) were assessed on day seven post MCAO. Results: Administration of EVs improved the sensorimotor function (p < 0.001) and reduced the apoptotic rate of Bax/Bcl-2 ratio (p < 0.001), as well as caspases and TUNEL-positive cells (p < 0.001) in comparison to the MCAO group. EV treatment also reduced the number of dead neurons and increased the number of MAP2+ cells in the IBZ (p < 0.001), as compared to the MCAO group. Conclusion: Our findings showed that HUCPVCs-derived EVs are more effective than their mother’s cells in improving neural function, possibly via the regulation of apoptosis in the ischemic rats. The strategy of cell-free extracts is, thus, helpful in removing the predicaments surrounding cell therapy in targeting brain diseases.

Item Type: Article
Keywords: Apoptosis, Extracellular vesicles, Ischemia, Middle cerebral artery
Subjects: QS Human Anatomy > QS504-532 Histology
QS Human Anatomy > QS604-681 Embryology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Histology and Anatomy
Page Range: pp. 342-355
Journal or Publication Title: Iranian Biomedical Journal
Journal Index: Pubmed, Scopus
Volume: 24
Number: 6
Publisher: Pasteur Institute of Iran
Identification Number: 10.29252/ibj.24.6.342
ISSN: 1028852X
Depositing User: دکتر علیرضا غریب
URI: http://eprints.muk.ac.ir/id/eprint/4662

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