Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Sensitization of MDA-MBA231 breast cancer cell to docetaxel by myricetin loaded into biocompatible lipid nanoparticles via sub-G1 cell cycle arrest mechanism

(2020) Sensitization of MDA-MBA231 breast cancer cell to docetaxel by myricetin loaded into biocompatible lipid nanoparticles via sub-G1 cell cycle arrest mechanism. Naunyn-Schmiedeberg's Archives of Pharmacology.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The harmful dose-dependent side effects of chemotherapy drugs have caused the discovery of novel perspective to evaluate chemotherapy protocols. In this study, the potential application of Compritol was investigated as a major scaffold into nanostructured lipid careers to highlight myricetin efficiency in treatment of breast cancer cells along with codelivery of docetaxel (DXT). Characterization of myricetin-loaded NLCs was carried out by measuring the particle size and zeta potential, using the scanning electron microscopy. MTT, DAPI staining, flow cytometric, and RT-PCR (real-time) assays were used to recognize novel formulation behavior on cell cytotoxicity as well as recognizing molecular mechanism of formulation concerning apoptosis phenomenon. Myricetin-loaded NLCs reduced the cell viability from 50 ± 2.3 to 40 ± 1.3% (p < 0.05). Percentage of apoptosis improved with combination treatment of myricetin-loaded NLCs and DXT in the MDA-MBA231 breast cancer cells. Expression of antiapoptotic genes (survivin, Cyclin B1, and Mcl1) indicated a significant reduction in factor along with increment in proapoptotic factor Bax and Bid mRNA rates. Overall, our results represented that the NLC delivery system could be a promising strategy to enhance the effect of anticancer agents such as DXT on breast cancer. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type: Article
Keywords: Antineoplastic Agents; Apoptosis; Breast Neoplasms Cell Line; Tumor Cell; Survival; Docetaxel Drug Carriers Drug Liberation Fatty Acids Flavonoids G1 Phase Cell Cycle Checkpoints Humans Nanoparticles
Subjects: WF Respiratory System > WF 140-900 Diseases of the Respiratory System
Divisions: Research Vice-Chancellor Department > Lung Diseases and Allergy Research Center
Journal or Publication Title: Naunyn-Schmiedeberg's Archives of Pharmacology
Journal Index: ISI, Pubmed, Scopus
Volume: 393
Number: 1
Publisher: Springer
Identification Number: 10.1007/s00210-019-01692-5
ISSN: 00281298
Depositing User: دکتر علیرضا غریب
URI: http://eprints.muk.ac.ir/id/eprint/4682

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