(2020) Effect of hypoxia preconditioned adipose-derived mesenchymal stem cell conditioned medium on cerulein-induced acute pancreatitis in mice. Advanced Pharmaceutical Bulletin.
|
Text
apb-10-297.pdf Download (2MB) | Preview |
Abstract
Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using the paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice. Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μg/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 μL/mice/30 min × 3 times). Twelve hours following the treatment, serum levels of amylase and lipase were measured. In addition, pancreas pathological changes, immunohistochemical examinations for evaluation of IL-6 expression and pancreatic myeloperoxidase (MPO) enzyme activity were analyzed. Results: The in vitro results of the morphological, differentiation and immunophenotyping analyses confirmed that hypoxia preconditioned MSCs (HP-MSCs) conserve MSCs characteristics after preconditioning. However, HP-MSCs significantly expressed high mRNA level of hypoxia inducible factor 1-α and higher level of total protein. The in vivo findings of the current study showed that CM and HCM significantly reduced the amylase & lipase activity, the severity of pancreas tissue injury and the expression of IL-6 and MPO enzyme activity compared with the AP group. However, no significant difference between CM and HCM groups was demonstrated. Conclusion: Use of CM and HCM can attenuate cerulein-induced AP and decrease inflammation in the pancreas tissue in AP mice.
Item Type: | Article |
---|---|
Keywords: | • Inflammation • Acute pancreatitis • Cerulein • Mesenchymal stem cell • Conditioned medium • Preconditioning |
Subjects: | QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics |
Divisions: | Research Vice-Chancellor Department > Student Research Committee |
Page Range: | pp. 297-306 |
Journal or Publication Title: | Advanced Pharmaceutical Bulletin |
Journal Index: | ISI, Pubmed, Scopus |
Volume: | 10 |
Number: | 2 |
Publisher: | Tabriz University of Medical Sciences |
Identification Number: | 10.34172/apb.2020.036 |
ISSN: | 22285881 |
Depositing User: | دکتر علیرضا غریب |
URI: | http://eprints.muk.ac.ir/id/eprint/4690 |
Actions (login required)
View Item |