Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice

(2016) Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice. Journal of Immunotoxicology.

Full text not available from this repository.

Official URL:


Estrogen is a neuro-protective hormone in various central nervous system (CNS) disorders. The present study evaluated the role of estrogen during experimental autoimmune encephalomyelitis (EAE) at doses selected to mimic any suppressive potential from the hormone during pregnancy. Here, mice were ovariectomized and then 2 weeks later treated with MOG antigen to induce EAE. Concurrently, mice then received (subcutaneously) an implanted pellet to deliver varying estrogen amounts over a 21-day period. Clinical scores and other parameters were monitored daily for the 21 days. At the end of the period, brain/spinal cord histology was performed to measure lymphocyte infiltration; T-cell profiles were determined through ELISA, flow cytometry, and real-time PCR. Transcription factor expression levels in the CNS were assessed using real-time PCR; T-cell differentiation was evaluated via flow cytometry. The results demonstrated that estrogen inhibited development of EAE. Histological studies revealed limited leukocyte infiltration into the CNS. High and medium dose of estrogen increased TH2 and Treg cell production of interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-β, but concurrently resulted in a significant reduction in production of interferon (IFN)-γ, IL-17, and IL-6. Flow cytometry revealed there were also significant decreases in the percentages of TH1 and TH17 cells, as well as significant increase in percentages of Treg and TH2 cells in the spleen and lymph nodes. Real-time PCR results indicated that high- and medium-dose estrogen treatments reduced T-bet and ROR-γt factor expression, but enhanced Foxp3 and GATA3 expression. Collectively, these results demonstrated that a medium dose of estrogen�similar to a pregnancy level of estrogen�could potentially reduce the incidence and severity of autoimmune EAE and possibly other autoimmune pathologies. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Keywords: estrogen; gamma interferon; interleukin 10; interleukin 17; interleukin 23; interleukin 4; interleukin 6; retinoid related orphan receptor gamma; transcription factor FOXP3; transcription factor GATA 3; transforming growth factor beta; cytokine; estrogen; transcription factor, animal experiment; animal model; animal tissue; Article; CD4+ T lymphocyte; controlled study; cytokine release; disease severity; down regulation; drug megadose; enzyme linked immunosorbent assay; estrogen blood level; estrogen therapy; experimental autoimmune encephalomyelitis; female; flow cytometry; histopathology; low drug dose; lymphocyte proliferation; lymphocytic infiltration; mouse; nonhuman; ovariectomy; priority journal; protein expression; real time polymerase chain reaction; T lymphocyte; Th1 cell; Th17 cell; treatment duration; animal; C57BL mouse; cell culture; dose calculation; experimental autoimmune encephalomyelitis; genetics; human; immunological tolerance; immunology; metabolism; multiple sclerosis; pregnancy; regulatory T lymphocyte; Th2 cell, Animals; Cells, Cultured; Cytokines; Drug Dosage Calculations; Encephalomyelitis, Autoimmune, Experimental; Estrogens; Female; Humans; Immune Tolerance; Mice; Mice, Inbred C57BL; Multiple Sclerosis; Ovariectomy; Pregnancy; T-Lymphocytes, Regulatory; Th2 Cells; Transcription Factors
Page Range: pp. 885-896
Journal or Publication Title: Journal of Immunotoxicology
Volume: 13
Number: 6
Publisher: Taylor and Francis Ltd
Identification Number: 10.1080/1547691X.2016.1223768
ISSN: 1547691X
Depositing User: مهندس جمال محمودپور

Actions (login required)

View Item View Item