(2016) The influence of lecithin administration on hepatic expression of FMO3 and FMO5 genes in N-mary mice. Biosciences Biotechnology Research Asia.
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Abstract
To investigate the effect of lecithin on FMO3 and FMO5 gene expression, L-α- lecithin or ethanol 30 (as control) was administrated to female N-mary mice as intraperitoneal injection and animals were sacrificed 48h after treatment. The relative expression of mRNA and protein were assessed by real-time PCR and western blotting analysis. In this study, FMO5 expression was also evaluated in HepG2 cell line. FMO3 mRNA expression in mice liver was increased to 1.8-fold in animal received lecithin compared to control group, While FMO5 expression did not showed any changes as in vivo and in vitro experiments. The our results suggest to induce of FMO3 expression by lecithin.
| Item Type: | Article |
|---|---|
| Keywords: | alcohol; complementary DNA; dimethylaniline monooxygenase; hypoxanthine phosphoribosyltransferase; phosphatidylcholine; trimethylamine, animal tissue; Article; controlled study; drug mechanism; female; FMO3 gene; FMO5 gene; gene; gene expression; human; human cell; mouse; mRNA expression assay; MTT assay; nonhuman; protein expression; real time polymerase chain reaction; RNA isolation; Western blotting |
| Page Range: | pp. 1797-1803 |
| Journal or Publication Title: | Biosciences Biotechnology Research Asia |
| Volume: | 13 |
| Number: | 3 |
| Publisher: | Oriental Scientific Publishing Company |
| Identification Number: | 10.13005/bbra/2332 |
| ISSN: | 09731245 |
| Depositing User: | مهندس جمال محمودپور |
| URI: | http://eprints.muk.ac.ir/id/eprint/498 |
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