Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Risk factors associated with community-acquired CTX-M producing Klebsiella pneumoniae typing by Rep-PCR in Sanandaj, Iran

(2016) Risk factors associated with community-acquired CTX-M producing Klebsiella pneumoniae typing by Rep-PCR in Sanandaj, Iran. Biosciences Biotechnology Research Asia.

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The CTX-M producing organism is emerging as a resistance source to oxyiminocephalosporin like ceftriaxone and ceftazidime. However, the laboratory detection of this gene has not yet been well defined in Sanandaj, Iran. The purpose beyond this study is identifying the prevalence of CTX-M and its risk factors in the community-acquired Klebsiella pneumoniae infections in Sanandaj. In this case-control study, 100 communityacquired Klebsiella pneumoniae strains were used. CTX-M gene was detected using PCR. The probable clonal relation among strains was determined via Rep-PCR. Risk factors associated with CTX-M positive were examined through the univariate logistic regression, Student's t-test, and Mann-Whitney U test. The polymerase chain reaction test, used to identify CTX-M gene, showed that 37 (37) Samples, out of 100 were positive. Based on Rep-PCR, 31 genotypes were identified among 37 Samples of CTX-M positive isolates. According to the statistical analysis, the following were the most important independent risk factors in this study: Gestation (p value= 0.036), previous exposure to antibiotic within 3 months (p value= 0.016), having relatives who work in a hospital (p value= 0.001, and distance of under 2 Km from home address to hospital (p value < 0.001). Regarding the increased value of ESBLs-producing strain, it is strongly recommended to use appropriate curative protocols based on the antibiogram. The results of Rep-PCR experiment refute the hypothesis of the clonal spread of one epidemic strain Klebsiella; meaning that not all CTX-M-producing species originate from the same strain and that the gene has extended among various strains. Hence, hospitals and their worker have to have better hygiene, hospital wastes have to be disposed properly, and antibiotics use may help prevent the spread of ESBL resistance only in case of being prescribed by a doctor.

Item Type: Article
Keywords: ceftazidime; ceftriaxone; extended spectrum beta lactamase, adult; Article; bacterium identification; case control study; clinical article; community acquired infection; controlled study; CTX-M gene; disk diffusion; extended spectrum beta lactamase producing Klebsiella pneumoniae; female; gene; hospitalization; human; Klebsiella pneumoniae; male; middle aged; nonhuman; phylogenetic tree; polymerase chain reaction; questionnaire; repetitive element sequence based polymerase chain reaction; risk factor; urinary catheter
Page Range: pp. 1311-1317
Journal or Publication Title: Biosciences Biotechnology Research Asia
Volume: 13
Number: 3
Publisher: Oriental Scientific Publishing Company
Identification Number: 10.13005/bbra/2271
ISSN: 09731245
Depositing User: مهندس جمال محمودپور

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