Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Class 1 integrons in non-clonal multidrugresistant acinetobacter baumannii from Iran, description of the new blaIMP-55 allele in in1243

(2016) Class 1 integrons in non-clonal multidrugresistant acinetobacter baumannii from Iran, description of the new blaIMP-55 allele in in1243. Journal of Medical Microbiology.

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Infections and outbreaks caused by multidrug-resistant Acinetobacter baumannii (MDR-AB) are prevalent and have been reported worldwide over the past 20 or more years. Class 1 integron in MDR-AB plays an important role in the spread of antibiotic resistance in clinical settings. This study has been conducted to evaluate the detection of metallo-β-lactamase, characterization of class 1 integron and determination of clonal relatedness among A. baumannii hospital isolates. Sixty-five clinical isolates of MDR-AB were recovered from two Iranian hospital’s intensive care units from February to August 2013. Integrase (intI1) and blaIMP genes were detected in 70.8 (n=46/65) and 9.23 (n=6/65) of the isolates using PCR assay, respectively. No other metallo-β-lactamase genes (blaVIM, blaSIM and blaNDM) were detected. PCR sequencing of integron gene cassette revealed the following arrays: blaOXA10-aacA4-blaIMP-55-cmlA5 (as a novel array was designated In1243), aacC1 and aadA1. Analysis of blaIMP gene revealed a new allele designated as blaIMP-55. Gene transfer experiment by conjugation showed the 36 kb conjugative plasmid harbouring In1243. The clonal assessment by repetitive extragenic palindromic PCR demonstrated a high-degree relatedness among the strains, but strains harbouring In1243 displayed a different repetitive extragenic palindromic PCR profile. In this study, we found that a novel class 1 integron (In1243) that encoded a new blaIMP allele resided on a transferable plasmid in non-clonal strains of MDR-AB. © 2016 The Authors.

Item Type: Article
Keywords: amikacin; aminoglycoside antibiotic agent; amoxicillin plus clavulanic acid; bacterial DNA; bacterial enzyme; cefixime; cefotaxime; ceftazidime; cephalosporin; ciprofloxacin; DNA fragment; gentamicin; integrase; integrase 1; levofloxacin; metallo beta lactamase; nalidixic acid; piperacillin; piperacillin plus tazobactam; rifampicin; tetracycline; tigecycline; tobramycin; unclassified drug; bacterial protein; beta lactamase; carbapenemase, Acinetobacter baumannii; Acinetobacter infection; allele; antibiotic resistance; antibiotic sensitivity; antibiotic therapy; Article; bacterial gene; bacterium conjugation; bacterium detection; bacterium isolate; bla imp 55 gene; clonal variation; controlled study; disk diffusion; gene cassette; hospitalization; human; integron; intensive care unit; Iran; length of stay; minimum inhibitory concentration; multidrug resistance; nonhuman; phenotype; plasmid; polymerase chain reaction; priority journal; Pseudomonas aeruginosa; sequence analysis; ultraviolet spectrophotometry; Acinetobacter baumannii; Acinetobacter infection; adult; allele; classification; DNA fingerprinting; DNA sequence; enzymology; female; genetics; genotype; horizontal gene transfer; hospital; isolation and purification; male; microbiology; middle aged; molecular typing, Acinetobacter baumannii; Acinetobacter Infections; Adult; Alleles; Bacterial Proteins; beta-Lactamases; Conjugation, Genetic; DNA Fingerprinting; Drug Resistance, Multiple, Bacterial; Female; Gene Transfer, Horizontal; Genotype; Hospitals; Humans; Integrons; Iran; Male; Middle Aged; Molecular Typing; Polymerase Chain Reaction; Sequence Analysis, DNA
Page Range: pp. 928-936
Journal or Publication Title: Journal of Medical Microbiology
Volume: 65
Number: 9
Publisher: Microbiology Society
Identification Number: 10.1099/jmm.0.000315
ISSN: 00222615
Depositing User: مهندس جمال محمودپور

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