Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

DNA fingerprinting and antimicrobial susceptibility pattern of clinical and environmental Acinetobacter baumannii isolates: a multicentre study

(2016) DNA fingerprinting and antimicrobial susceptibility pattern of clinical and environmental Acinetobacter baumannii isolates: a multicentre study. Journal of Chemotherapy.

Full text not available from this repository.

Official URL:


Abstract: Background and aim: The aims of this study were to establish antibiotic profile and the molecular epidemiology of Acinetobacter baumannii isolates, with considering the effectiveness of control infection measures across three hospitals in the Kurdistan, west part of Iran. Methods: Fifty-four A. baumannii isolates were collected from patients and environmental specimens. Antibiotic susceptibility patterns (Antibio-type) were evaluated for 17 different antibiotics and MIC for imipenem was done. Isolates were assessed for the presence of metallo-beta-lactamases (MBLs), class 1 and 2 integrons, and integrated gene cassettes and blaOXA-likefamilies genes. Repetitive-sequence-based PCR (REP-PCR) was done for analysing clonality and relativeness of isolates (REP-type). Results: Antibiotic susceptibility patterns distinguished 11 distinct Antibio-types and REP-PCR showed three clusters with 20 subclusters, mostly belonged to two clonal subgroups, A1 and B1. blaOXA-51 and blaOXA-23 were detected in 100 (54/54) and 52 (28/54), respectively, while blaOXA-24-like and blaOXA-58 were not present in isolates. MBLs were not detected, but, however, high rate of imipenem resistance was observed (52). MIC90 of imipenem was 16 mg/ml. Class 1 integrons were detected in 11 (6/54) of isolates followed by 24 (13/54) of class 2. Both classes of integron genes were detected in 15 (8/54) of isolates. Integrated gene cassettes were in low level (11 of class 1 harboring isolates). Two arrays of gene cassettes were revealed, dfrA5-like and dfrA17-aadA5. Conclusion: Infection control surveillance should be considered as a serious manner, even the superficial eradication of hospital acquired pathogens. MBL genes were not induced carbapenem resistance in studied hospital settings, but blaOXA-51 & 23 contributed in imipenem resistant. Integrons had a little share in resistance of A. baumannii isolates. © 2016 Edizioni Scientifiche per l'Informazione su Farmaci e Terapia.

Item Type: Article
Keywords: amikacin; aminoglycoside; antibiotic agent; carbapenem; cefepime; cefotaxime; ceftazidime; ceftriaxone; cephalosporin; ciprofloxacin; colistin; cotrimoxazole; cytochrome c oxidase; gentamicin; imipenem; meropenem; metallo beta lactamase; norfloxacin; ofloxacin; penicillin derivative; piperacillin; quinoline derived antiinfective agent; sultamicillin; tobramycin; beta lactamase, Acinetobacter baumannii; Acinetobacter calcoaceticus-baumannii complex; Acinetobacter infection; antibiotic sensitivity; Article; bronchoscopy; controlled study; cross-sectional study; disk diffusion; DNA fingerprinting; DNA sequence; Escherichia coli; gene amplification; gene cassette; hospital infection; human; infection control; inhibition zone; integron; major clinical study; minimum inhibitory concentration; nonhuman; nucleotide sequence; polymerase chain reaction; Pseudomonas aeruginosa; urinalysis; Acinetobacter Infections; antibiotic resistance; classification; clinical trial; cross infection; DNA fingerprinting; drug effects; genetics; Iran; isolation and purification; microbiology; multicenter study, Acinetobacter baumannii; Acinetobacter Infections; beta-Lactamases; Cross Infection; Cross-Sectional Studies; DNA Fingerprinting; Drug Resistance, Bacterial; Environmental Microbiology; Humans; Infection Control; Integrons; Iran
Page Range: pp. 277-283
Journal or Publication Title: Journal of Chemotherapy
Volume: 28
Number: 4
Publisher: Taylor and Francis Ltd.
Identification Number: 10.1080/1120009X.2016.1175706
ISSN: 1120009X
Depositing User: مهندس جمال محمودپور

Actions (login required)

View Item View Item