Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Effects of Pistacia atlantica subsp. Kurdica on growth and aflatoxin production by aspergillus parasiticus

(2016) Effects of Pistacia atlantica subsp. Kurdica on growth and aflatoxin production by aspergillus parasiticus. Jundishapur Journal of Microbiology.

Full text not available from this repository.

Official URL:


Background: Aflatoxins are highly toxic secondary metabolites mainly produced by Aspergillus parasiticus. This species can contaminate a wide range of agricultural commodities, including cereals, peanuts, and crops in the field. In recent years, research on medicinal herbs, such as Pistacia atlantica subsp. kurdica, have led to reduced microbial growth, and these herbs also have a particular effect on the production of aflatoxins as carcinogenic compounds. Objectives: In this study, we to examine P. atlantica subsp. kurdica as a natural compound used to inhibit the growth of A. parasiticus and to act as an anti-mycotoxin. Materials and Methods: In vitro antifungal susceptibility testing of P. atlantica subsp. kurdica for A. parasiticus was performed according to CLSI document M38-A2. The rate of aflatoxin production was determined using the HPLC technique after exposure to different concentrations (62.5 - 125 mg/mL) of the gum. The changes in expression levels of the aflR gene were analyzed with a quantitative real-time PCR assay. Results: The results showed that P. atlantica subsp. kurdica can inhibit A. parasiticus growth at a concentration of 125 mg/mL. HPLC results revealed a significant decrease in aflatoxin production with 125 mg/mL of P. atlantica subsp. kurdica, and AFL-B1 production was entirely inhibited. Based on quantitative real-time PCR results, the rate of aflR gene expression was significantly decreased after treatment with P. atlantica subsp. kurdica. Conclusions: Pistacia atlantica subsp. kurdica has anti-toxic properties in addition to an inhibitory effect on A. parasiticus growth, and is able to decrease aflatoxin production effectively in a dose-dependent manner. Therefore, this herbal extract maybe considered a potential anti-mycotoxin agent in medicine or industrial agriculture. © 2016, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Keywords: aflatoxin; aflatoxin B1; aflatoxin B2; aflatoxin G1; aflatoxin G2; antifungal agent; natural product, aflR gene; antifungal activity; antifungal susceptibility; Article; Aspergillus parasiticus; concentration response; controlled study; fungal gene; fungus growth; gene expression; high performance liquid chromatography; in vitro study; minimum inhibitory concentration; nonhuman; Pistacia; Pistacia atlantica subsp. kurdica; quantitative analysis; real time polymerase chain reaction; toxin synthesis
Journal or Publication Title: Jundishapur Journal of Microbiology
Volume: 9
Number: 7
Publisher: Kowsar Medical Publishing Company
Identification Number: 10.5812/jjm.35452
ISSN: 20083645
Depositing User: مهندس جمال محمودپور

Actions (login required)

View Item View Item