Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Feeding role of mouse embryonic fibroblast cells is influenced by genetic background, cell passage and day of isolation

(2022) Feeding role of mouse embryonic fibroblast cells is influenced by genetic background, cell passage and day of isolation. Zygote (Cambridge, England).

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/35485762

Abstract

Mouse embryonic fibroblast (MEF) cells are commonly used as feeder cells to maintain the pluripotent state of stem cells. MEFs produce growth factors and provide adhesion molecules and extracellular matrix (ECM) compounds for cellular binding. In the present study, we compared the expression levels of Fgf2, Bmp4, ActivinA, Lif and Tgfb1 genes at the mRNA level and the level of Fgf2 protein secretion and Lif cytokine secretion at passages one, three and five of MEFs isolated from 13.5-day-old and 15.5-day-old embryos of NMRI and C57BL/6 mice using real-time PCR and enzyme-linked immunosorbent assay. We observed differences in the expression levels of the studied genes and secretion of the two growth factors in the three passages of MEFs isolated from 13.5-day-old and 15.5-day-old embryos, respectively. These differences were also observed between the NMRI and C57BL/6 strains. The results of this study suggested that researchers should use mice embryos that have different genetic backgrounds and ages, in addition to different MEF passages, when producing MEFs based on the application and type of their study.

Item Type: Article
Keywords: Animals Cell Differentiation Cells, Cultured Feeder Cells/metabolism *Fibroblast Growth Factor 2/genetics/metabolism *Fibroblasts Genetic Background Mice Mice, Inbred C57BL Fibroblast growth factor-2 (Fgf2) Inbred mice Leukaemia inhibitory factor (Lif) Mouse embryonic fibroblasts (MEFs) Outbred strain
Page Range: pp. 550-560
Journal or Publication Title: Zygote (Cambridge, England)
Volume: 30
Number: 4
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/5569

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