Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Characterization of Escherichia coli isolated from urinary tract infection and association between virulence expression and antimicrobial susceptibility

(2022) Characterization of Escherichia coli isolated from urinary tract infection and association between virulence expression and antimicrobial susceptibility. BMC microbiology.

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/35387587

Abstract

BACKGROUND: The capacity of antibiotics to modulate bacterial virulence has raised concerns over the appropriateness of antibiotic therapies, including when dosing strategies fall below sub-therapeutic levels. In this work, we investigated the ability of antibiotics to influence virulence in Escherichia coli isolated from urinary tract infection (UTI). RESULTS: Out of 120 isolates, 32.5 carried pap, 21.7 carried hlyA, and 17.5 carried cnf. The predominant B2 phylogroup was significantly associated with the quinolone-resistant isolates. A significant association was seen between the presence of hlyA hemolysin and susceptibility to ceftriaxone and ciprofloxacin (P < 0.05). Sub-inhibitory concentrations of both antibiotics reduced the levels of hlyA expression and hemolysis in isolates treated with antibiotics compared to untreated isolates (P < 0.05). Growth rate assay showed that the decrease in hlyA expression was not an effect of decreased growth rate. CONCLUSION: Our study indicated the inhibitory effect of ciprofloxacin and ceftriaxone on the level of hemolysis, suggesting that the sub-inhibitory concentrations of these antibiotics may affect the outcome of infections. Further studies, including animal models may elucidate the outcome of virulence modulation by these antibiotics in UTI pathogenesis.

Item Type: Article
Keywords: Animals Anti-Bacterial Agents/pharmacology/therapeutic use Ceftriaxone/pharmacology Ciprofloxacin/pharmacology Escherichia coli *Escherichia coli Infections/microbiology Hemolysis *Urinary Tract Infections/microbiology Virulence Virulence Factors/genetics Gene expression Hemolysin Minimum inhibitory concentration Pathogenicity
Page Range: p. 89
Journal or Publication Title: BMC microbiology
Volume: 22
Number: 1
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/5584

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