Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Captopril and Spironolactone can Attenuate Diabetic Nephropathy in Wistar Rats by Targeting ABCA1 and microRNA-33

(2022) Captopril and Spironolactone can Attenuate Diabetic Nephropathy in Wistar Rats by Targeting ABCA1 and microRNA-33. Current pharmaceutical design.

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BACKGROUND: Nephropathy diabetes is one of the important causes of death and a more prevalent cause of end-stage renal disease. OBJECTIVE: The present study investigated the effect of applying spironolactone and captopril and their combination on some renal performance indices and cholesterol-efflux-related gene expression in nephropathy diabetic rats. METHODS: Intraperitoneal injection of streptozotocin was used to induce diabetes in rats. FBS, creatinine, and BUN were assayed using the calorimetry technique; also, urine microalbumin was assayed by ELISA. Hepatic gene expressions of ABCA1, ABCG1, and miR-33 were evaluated by the real-time PCR method. RESULTS: FBS levels in the captopril-treated group were significantly decreased compared with the untreated diabetic group. BUN levels of treated groups with captopril and a combination of captopril + spironolactone were significantly increased. GFR of both treated diabetic groups with captopril and spironolactone was significantly lower than an untreated diabetic group. ABCA1 gene expression in hepatic cells of the combination of spironolactone + captopril treated group was significantly increased compared to other treated and untreated diabetic groups. The hepatic expression of the ABCG1 gene in the treated and untreated diabetic groups was significantly lower than in the control group. Treatment of the diabetic group with only combination therapy decreased the hepatic gene expression of miR-33 significantly. CONCLUSION: Obtained results suggest that S+C combination therapy can improve nephropathy and diabetes disorders by targeting the ABCA1 and miR-33 gene expression. It is suggested that miR-33 and ABCA1 genes evaluation could be a new therapeutic strategy for nephropathy diabetes remediation.

Item Type: Article
Keywords: ATP Binding Cassette Transporter 1 Animals Captopril/metabolism/pharmacology/therapeutic use *Diabetes Mellitus, Experimental/drug therapy/metabolism *Diabetic Nephropathies/drug therapy Kidney *MicroRNAs/metabolism Rats Rats, Wistar Spironolactone/metabolism/pharmacology/therapeutic use Abca1 Abcg1 Diabetes captopril microRNA nephropathy spironolactone
Page Range: pp. 1367-1372
Journal or Publication Title: Current pharmaceutical design
Volume: 28
Number: 16
Depositing User: مهندس مهدی شریفی

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