Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

High prevalence of clinical and environmental triazole-resistant Aspergillus fumigatus in Iran: Is it a challenging issue?

(2016) High prevalence of clinical and environmental triazole-resistant Aspergillus fumigatus in Iran: Is it a challenging issue? Journal of Medical Microbiology.

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Triazole antifungal agents are the mainstay of aspergillosis treatment. As highlighted in numerous studies, the global increase in the prevalence of triazole resistance could hamper the management of aspergillosis. In the present three-year study, 513 samples (213 clinical and 300 environmental samples) from 10 provinces of Iran were processed and screened in terms of azole resistance (4 and 1 mg l�1 of itraconazole and voriconazole, respectively), using selective plates. Overall, 150 A. fumigatus isolates (71 clinical and 79 environmental isolates) were detected. The isolates were confirmed by partial sequencing of the β-tubulin gene. Afterwards, in vitro antifungal susceptibility tests against triazole agents were performed, based on the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. The CYP51A gene was sequenced in order to detect mutations. The MIC of itraconazole against 10 (6.6) strains, including clinical (n=3, 4.2) and environmental (n=7, 8.8) strains, was higher than the breakpoint and epidemiological cut-off value. Based on the findings, the prevalence of azole-resistant A. fumigatus in Iran has increased remarkablyfrom 3.3 to 6.6 in comparison with earlier epidemiological research. Among resistant isolates, TR34/L98H mutations in theCYP51A gene were the most prevalent (n=8, 80), whereas other point mutations (F46Y, G54W, Y121F, G138C, M172V, F219C, M220I, D255E, T289F, G432C and G448S mutations) were not detected. Although the number of patients affected by azole-resistant A. fumigatus isolates was limited, strict supervision of clinical azole-resistant A. fumigatus isolates and persistent environmental screening of azole resistance are vital to the development of approaches for the management of azole resistance in human pathogenic fungi. © 2016 The Authors.

Item Type: Article
Keywords: amphotericin B; beta tubulin; caspofungin; itraconazole; posaconazole; pyrrole; triazole; voriconazole; antifungal agent; cytochrome P-450 CYP51A, Aspergillus; cytochrome P450; fungal protein; itraconazole; voriconazole, antifungal resistance; antifungal susceptibility; Article; Aspergillus fumigatus; CYP51A gene; fungal gene; fungus isolation; gene mutation; gene sequence; in vitro study; Iran; minimum effective concentration; minimum inhibitory concentration; nonhuman; prevalence; priority journal; aspergillosis; Aspergillus fumigatus; dna mutational analysis; drug effects; gene expression regulation; genetics; human; metabolism; microbiology; multidrug resistance; mutation, Antifungal Agents; Aspergillosis; Aspergillus fumigatus; Cytochrome P-450 Enzyme System; DNA Mutational Analysis; Drug Resistance, Multiple, Fungal; Fungal Proteins; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Humans; Iran; Itraconazole; Mutation; Prevalence; Voriconazole
Page Range: pp. 468-475
Journal or Publication Title: Journal of Medical Microbiology
Volume: 65
Number: 6
Publisher: Microbiology Society
Identification Number: 10.1099/jmm.0.000255
ISSN: 00222615
Depositing User: مهندس جمال محمودپور

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