Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV-HBV and HIV-HCV Coinfections

(2016) Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV-HBV and HIV-HCV Coinfections. Journal of Clinical Laboratory Analysis.

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Background: CD4+ cell count, the common HIV infection screening test, is costly and unable to differentiate HIV monoinfection from its concurrent infection with hepatitis B or C virus. We aimed to ascertain diagnostic value of serum adenosine deaminase (ADA) activity as a useful tool to differentiate HIV mono- and co-infection. Methods: Blood samples were collected from 30 HIV-HBV and 30 HIV-HCV coinfected patients, 33 HIV positive subjects, and 72 controls. CD4+ cell count, serum total ADA (tADA), and ADA1, and ADA2 isoenzyme activities were determined and their sensitivity and specificity were computed. Results: tADA and ADA2 activities were significantly higher and CD4+ counts were markedly lower in all patients compared with controls. Strong inverse agreements between CD4+ cell counts and both tADA and ADA2 activities were observed. Serum tADA and ADA1 activities showed the highest specificity and the highest sensitivity, respectively, for differentiating HIV monoinfection from HIV-HBV and HIV-HCV coinfections. Conclusions: We showed strong agreement and correlation between CD4+ cell count and ADA enzyme activity. Based on high ADA sensitivity and specificity, it is concluded that determination of ADA activity might be a novel diagnostic tool to distinguish of HIV monoinfection from its coinfection with HBV or HCV. © 2016 Wiley Periodicals, Inc.

Item Type: Article
Keywords: adenosine deaminase; adenosine deaminase 1 isoenzyme; adenosine deaminase 2 isoenzyme; unclassified drug; adenosine deaminase, adult; area under the curve; Article; blood sampling; CD4 lymphocyte count; controlled study; diagnostic value; differential diagnosis; disease course; enzyme activity; enzyme blood level; female; hepatitis B; hepatitis C; human; human cell; Human immunodeficiency virus infection; infection; major clinical study; male; middle aged; mixed infection; monoinfection; receiver operating characteristic; sensitivity and specificity; young adult; blood; Coinfection; enzymology; hepatitis B; hepatitis C; HIV Infections, Adenosine Deaminase; Adult; Coinfection; Female; Hepatitis B; Hepatitis C; HIV Infections; Humans; Male; Middle Aged; Young Adult
Page Range: pp. 200-203
Journal or Publication Title: Journal of Clinical Laboratory Analysis
Volume: 30
Number: 3
Publisher: John Wiley and Sons Inc.
Identification Number: 10.1002/jcla.21836
ISSN: 08878013
Depositing User: مهندس جمال محمودپور

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