Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Quercetin elicits proapoptotic effect through downregulation of antiapoptotic and upregulation of proapoptotic genes in human myeloid leukemia kg-1 cells

(2021) Quercetin elicits proapoptotic effect through downregulation of antiapoptotic and upregulation of proapoptotic genes in human myeloid leukemia kg-1 cells. Annals of Cancer Research and Therapy.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily, triggering apoptosis in malignant cell without affecting healthy cells. However, varied types of the cancerous cells (e.g., leukemia) show resistance to TRAIL-induced apoptosis (TIA). Herein, we evaluated the effect of quercetin (QUE) in combination with TRAIL to defeat the resistance of the human acute myeloid leukemia KG-1 cell lines against TIA. Briefly, we treated the KG-1 cells with TRAIL (50 and 250 ng/ml) and QUE (100 μM) alone and in combination together. Then, the expression levels of the pro-apoptotic proteins, including caspase 3, 8, and 9 along with the anti-apoptotic proteins such as survivin and Mcl-1 expressions levels were evaluated by real-time PCR in KG-1 cells 12, 14 and 48 hours upon exposure with TRAIL and QUE (100 μM). Based on results, the combination of TRAIL and QUE augmented the expression levels of the caspase 3, 8, and 9 compared with the cells treated with TRAIL and QUE alone. Conversely, expression rates of the sur-vivin and Mcl-1 were strongly diminished in cells treated with TRAIL plus QUE in comparison to KG-1 cells that treated with TRAIL and QUE alone. Considering finding, QUE can not only improve caspase 3, 8, and 9 when used plus TRAIL, it also made a positive effect on their activation due to the inhibitory impacts on anti-apoptotic protein Mcl-1 and survivin expressions in KG-1 cells, possibly leading to the desired therapeutic outcome in leukemic cells. © 2021, PJD Publications Ltd. All rights reserved.

Item Type: Article
Keywords: Acute myeloid leukemia Apoptosis KG-1 cell line Quercetin TNF-related apoptosis-inducing ligand (TRAIL) baculoviral IAP repeat containing protein 5 caspase 3 caspase 8 caspase 9 death receptor 4 dimethyl sulfoxide penicillin derivative protein mcl 1 tumor necrosis factor tumor necrosis factor related apoptosis inducing ligand antiapoptotic activity Article cancer resistance controlled study down regulation drug combination fetal bovine serum gene expression human human cell myeloid leukemia cell line proapoptotic activity protein expression real time polymerase chain reaction RNA extraction ultraviolet spectroscopy upregulation
Page Range: pp. 96-101
Journal or Publication Title: Annals of Cancer Research and Therapy
Volume: 29
Number: 1
Depositing User: مهندس مهدی شریفی
URI: http://eprints.muk.ac.ir/id/eprint/6058

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