Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

Detection of Leishmania major DNA within wild caught Phlebotomus papatasi and species composition of sand flies in endemic focus of cutaneous leishmaniasis, in western Iran

(2016) Detection of Leishmania major DNA within wild caught Phlebotomus papatasi and species composition of sand flies in endemic focus of cutaneous leishmaniasis, in western Iran. Journal of Parasitic Diseases.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Cutaneous leishmaniasis is one of the most important public health problem in many developing countries. The present study was conducted to determine the vector(s), the parasite and the species composition of sand flies in the Dehloran County during May�November 2012. Sand flies were collected by sticky traps and mounted in Puri�s medium for species identification. Polymerase chain reaction (PCR) techniques of kDNA, ITS1-rDNA, followed by restriction fragment length polymorphism (RFLP) were used for identification of DNA of Leishmania parasites in infected sand flies. A total of 82443 specimens comprising 15 species of sand flies (5 Phlebotomus and 10 Sergentomyia) were collected and identified. The species of Phlebotomus papatasi was dominant in outdoor and indoor resting places. Among the 280 specimens of female P. papatasi tested by PCR of kDNA, ITS1-rDNA genes of the parasite followed by RFLP, only 5 of them (1.8 ) were positive to Leishmania major parasites. This is the first molecular detection of leishmania infection of P. papatasi to L. major in this region. The results indicated that, P. papatasi was only species found infected by L. major and the principal vector of disease agent to human. © 2014, Indian Society for Parasitology.

Item Type: Article
Keywords: internal transcribed spacer 1; kinetoplast DNA; protozoal DNA; ribosome DNA, Article; diagnostic kit; disease carrier; DNA extraction; endemic disease; female; Iran; Leishmania major; male; nonhuman; parasite identification; Phlebotominae; Phlebotomus papatasi; polymerase chain reaction; priority journal; restriction fragment length polymorphism; skin leishmaniasis; species composition
Page Range: pp. 69-74
Journal or Publication Title: Journal of Parasitic Diseases
Volume: 40
Number: 1
Publisher: Springer India
Identification Number: 10.1007/s12639-014-0448-0
ISSN: 09717196
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/606

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