Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

An optimised mouse model of chronic pancreatitis with a combination of ethanol and cerulein

(2016) An optimised mouse model of chronic pancreatitis with a combination of ethanol and cerulein. Central European Journal of Immunology.

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Introduction: Chronic pancreatitis (CP) is an intractable and multi-factorial disorder. Developing appropriate animal models is an essential step in pancreatitis research, and the best ones are those which mimic the human disorder both aetiologically and pathophysiologically. The current study presents an optimised protocol for creating a murine model of CP, which mimics the initial steps of chronic pancreatitis in alcohol chronic pancreatitis and compares it with two other mouse models treated with cerulein or ethanol alone. Material and methods: Thirty-two male C57BL/6 mice were randomly selected, divided into four groups, and treated intraperitoneally with saline (10 ml/kg, control group), ethanol (3 g/kg; 30 v/v), cerulein (50 μg/kg), or ethanol + cerulein, for six weeks. Histopathological and immunohistochemical assays for chronic pancreatitis index along with real-time PCR assessments for mRNA levels of inflammatory cytokines and fibrogenic markers were conducted to verify the CP induction. Results: The results indicated that CP index (CPI) was significantly increased in ethanol-cerulein mice compared to the saline, ethanol, and cerulein groups (p < 0.001). Interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), transforming growth factor β (TGF-β), α-smooth muscle actin (α-SMA), and myeloperoxidase activity were also significantly greater in both cerulein and ethanol-cerulein groups than in the saline treated animals (p < 0.001). Immunohistochemical analysis revealed enhanced expression of TGF-β and α-SMA in ethanol-cerulein mice compared to the saline group. Conclusions: Intraperitoneal (IP) injections of ethanol and cerulein could successfully induce CP in mice. IP injections of ethanol provide higher reproducibility compared to ethanol feeding. The model is simple, non-invasive, reproducible, and time-saving. Since the protocol mimics the initial phases of CP development in alcoholics, it can be used for investigating basic mechanisms and testing new therapies. © 2016 Termedia Sp. z o.o. All rights reserved.

Item Type: Article
Keywords: alcohol; alpha smooth muscle actin; ceruletide; interleukin 1beta; myeloperoxidase; sodium chloride; transforming growth factor beta; tumor necrosis factor alpha, animal experiment; animal model; animal tissue; Article; body weight; chronic pancreatitis; controlled study; enzyme activity; histopathology; immunohistochemistry; male; mouse; mouse model; nonhuman; protein expression; real time polymerase chain reaction
Page Range: pp. 54-63
Journal or Publication Title: Central European Journal of Immunology
Volume: 41
Number: 1
Publisher: Termedia Publishing House Ltd.
Identification Number: 10.5114/ceji.2016.58816
ISSN: 14263912
Depositing User: مهندس جمال محمودپور

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