Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

N-acetylcysteine prevents rotenone-induced Parkinson's disease in rat: An investigation into the interaction of parkin and Drp1 proteins

(2015) N-acetylcysteine prevents rotenone-induced Parkinson's disease in rat: An investigation into the interaction of parkin and Drp1 proteins. Brain Research Bulletin.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

There are convincing evidences that oxidative stress has an important role in both the initiation and progression of Parkinson's disease. N-acetylcysteine (NAC) is shown to have antioxidant properties via fortifying glutathione which is one of the main endogenous antioxidant systems. Therefore our study was aimed to evaluate the effect of NAC in management of Parkinson's disease. To this aim, male Wistar rats (10-12 months) received rotenone 2.5. mg/kg/48. h intraperitoneally (ip) to induce a Parkinson's disease model. Pretreatment with NAC (25 and 50. mg/kg/48. h ip) was administered 1. h before the rotenone injection. Three behavioral tests (rotarod, rearing and bar tests) were performed for motor function assessment. Dopamine levels of dopaminergic areas in rat brain including substantia nigra (SN) and striatum (ST) were assessed using high performance liquid chromatography analysis to measure the loss of dopamine. Western blot analysis was also done for parkin and Drp1 (dynamin related protein-1) proteins quantification in SN and ST. Our results indicated that NAC significantly ameliorated the rotenone-induced motor dysfunction and dopamine loss. Furthermore, NAC was able to prevent the rotenone-induced changes in parkin and Drp1 levels in the both studied areas. In conclusion we found that NAC delayed the Parkinson's disease induction by rotenone and this effect might be related to its proved antioxidant effect. © 2015 Elsevier Inc.

Item Type: Article
Keywords: acetylcysteine; dopamine; dynamin related protein 1; parkin; peptides and proteins; rotenone; unclassified drug; acetylcysteine; dopamine; Drp1 protein, rat; dynamin; parkin; protein binding; rotenone; ubiquitin protein ligase, animal experiment; animal model; animal tissue; antioxidant activity; Article; controlled study; corpus striatum; dopaminergic nerve cell; experimental behavioral test; high performance liquid chromatography; male; motor dysfunction; motor performance; muscle strength; neuroprotection; nonhuman; Parkinson disease; priority journal; protein expression; rat; substantia nigra; animal; antagonists and inhibitors; chemically induced; disease model; drug effects; drug interaction; metabolism; oxidative stress; Parkinsonian Disorders; randomization; rotarod test; Wistar rat, Acetylcysteine; Animals; Corpus Striatum; Disease Models, Animal; Dopamine; Dopaminergic Neurons; Drug Interactions; Dynamins; Male; Oxidative Stress; Parkinsonian Disorders; Protein Binding; Random Allocation; Rats; Rats, Wistar; Rotarod Performance Test; Rotenone; Substantia Nigra; Ubiquitin-Protein Ligases
Page Range: pp. 34-40
Journal or Publication Title: Brain Research Bulletin
Volume: 113
Publisher: Elsevier Inc.
Identification Number: 10.1016/j.brainresbull.2015.02.007
ISSN: 03619230
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/774

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