Repository of Research and Investigative Information

Repository of Research and Investigative Information

Kurdistan University of Medical Sciences

The effect of aminoguanidine on sperm motility and mitochondrial membrane potential in varicocelized rats

(2016) The effect of aminoguanidine on sperm motility and mitochondrial membrane potential in varicocelized rats. Iranian Journal of Basic Medical Sciences.

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Objective(s): Increased levels of nitric oxide (NO) in the testicular veins of people suffering from varicocele have already been reported. However, the role of NO-synthase (NOS) isozymes and their inhibitors have not been extensively studied. We aimed to evaluate the inhibitory effects of aminoguanidine (AG), on sperm motility, vitality, and mitochondrial membrane potential (MMP) in varicocelized rats. Materials and Methods: Twenty fore male Wister rats were divided into control, sham, varicocele, and treatment groups. Varicocele and treatment groups underwent partial ligation of left renal vein. Rats in the sham group underwent the same procedures as the varicocele group with the exception of vein ligation. 10 weeks after varicocele induction, sperm parameters were evaluated in all groups. The treatment group received 50 mg/kg AG injection daily for 10 weeks after which they were sacrificed prior to assessment of the parameters. Sperm viability and MMP were assessed by flow cytometry using propidium iodide (PI) and rhodamine 123 (Rh123), respectively. Results: The results of this study show a decrease in sperm viability, motility and MMP in the varicocele group compared with the other groups. After AG injection, we observed that all the parameters were significantly enhanced in the treatment group compared with the other groups. Rh123 staining revealed a positive relation between MMP and sperm motility, whereas PI staining showed a positive relation between sperm motility and viability. Conclusion: The findings of our study show that AG improves sperm motility and MMP, and thus, might be useful in the management of varicocele-related infertility. © 2016, Mashhad University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: aminoguanidine; nitric oxide; propidium iodide; rhodamine 123, adult; animal model; animal tissue; Article; cell viability; controlled study; flow cytometry; male; mitochondrial membrane potential; nonhuman; rat; spermatozoon; spermatozoon motility; varicocele
Page Range: pp. 1279-1284
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Volume: 19
Number: 12
Publisher: Mashhad University of Medical Sciences
Identification Number: 10.22038/ijbms.2016.7908
ISSN: 20083866
Depositing User: مهندس جمال محمودپور
URI: http://eprints.muk.ac.ir/id/eprint/444

Actions (login required)

View Item View Item